非综合征型唇腭裂中SMAD7和CD44表达水平及其临床意义

Expression levels of SMAD7 and CD44 in nonsyndromic orofacial clefts and its clinical significance

目的 探讨非综合征型唇腭裂(nonsyndromic orofacial clefts, NOC)组织中SMAD7及CD44的表达水平及临床意义。方法 选取20例唇裂伴或不伴腭裂组织(CL/P组),20例单纯腭裂组织(CPO组),15例健康对照组(C组),采用Western blot检测各组组织中SMAD7以及CD44的表达水平。结果 与健康对照组相比,CL/P组及CPO组中SMAD7、CD44的表达量下调,差异有统计学意义(P<0.05)。结论 SMAD7、CD44可能在NSOC的发生、发展中发挥作用,对NSOC的早期预防、诊断以及后期的治疗具有重要意义。

Objective To investigate the expression levels of SMAD7 and CD44 in nonsyndromic orofacial clefts and its clinical significance.Methods Twenty cleft lip with or without cleft palate tissues(group CL/P),twenty simple cleft palate tissues(group CPO)and fifteen healthy control group(group C)were selected,then the expression levels of SMAD7 and CD44 were detected by Western blot.Results Compared with the group C,the expression of SMAD7 and CD44 in group CL/P and group CPO were down-regulated and the difference was statistically significant(P<0.05).Conclusion SMAD7 and CD44 may play the role in the occurrence and development of NSOC,which is of great significance for the early prevention,diagnosis and later treatment of NSOC.