金华猪肌肉和血清氨基酸谱的发育性变化及其与肌肉生长的相关性

Developmental changes of amino acid profiles in muscle and serum and their correlation with muscle growth of Jinhua pigs

选择同批次的60头金华猪仔猪进行饲养,在45、90、150、270日龄分别选择4头金华猪进行屠宰,测定眼肌面积,并采集背最长肌和血清样品,分别用于水解氨基酸和游离氨基酸及其衍生物的测定。试验结果显示:随着日龄增加,金华猪眼肌面积显著(P<0.05)增大。肌肉水解氨基酸中:必需氨基酸含量和总氨基酸含量在45日龄至270日龄间先显著(P<0.05)升高,再显著(P<0.05)下降,之后基本保持稳定;非必需氨基酸含量先显著(P<0.05)升高,再显著(P<0.05)下降,后又显著(P<0.05)升高。血清游离氨基酸中:必需氨基酸含量在45日龄至270日龄间先保持稳定,最后显著(P<0.05)升高;非必需氨基酸含量先显著(P<0.05)降低,再显著(P<0.05)升高,后保持稳定;总氨基酸含量先显著(P<0.05)下降,之后显著(P<0.05)升高。肌肉和血清中的大部分氨基酸含量均与眼肌面积呈正相关,其中,精氨酸、缬氨酸、异亮氨酸和亮氨酸含量与眼肌面积呈较强的正相关,但甘氨酸含量与眼肌面积呈负相关。综上,金华猪肌肉和血清中的氨基酸谱呈现发育性动态变化,精氨酸、缬氨酸、异亮氨酸和亮氨酸含量变化与肌肉生长存在较强相关性。

In the present study,a total of 60 Jinhua piglets from the same batch were fed with commercial diets under the standard management.Four Jinhua pigs were randomly selected and slaughtered at 45,90,150 and 270 days of age,respectively,to determine the ribeye areas,and longissimus dorsi muscle and serum samples were collected to determine the content of amino acids and their derivatives.The results showed that the ribeye areas of Jinhua pigs significantly(P<0.05)increased with age.In the longissimus dorsi:essential amino acids content and total amino acids content significantly(P<0.05)increased first,then significantly(P<0.05)decreased and remained stable after;while nonessential amino acids content increased significantly(P<0.05)first,decreased significantly(P<0.05)later and then increased significantly(P<0.05).In the serum:essential amino acids content remained stable first,and then increased significantly(P<0.05);nonessential amino acids content decreased significantly(P<0.05)first,increased significantly(P<0.05)later and then remained stable;total amino acid content decreased significantly(P<0.05)first,then increased significantly(P<0.05).Most of the amino acids contents in muscle and serum were positively correlated with ribeye areas,where the contents of arginine,valine,isoleucine and leucine contents gave a strong positive correlation with ribeye areas,but glycine content in the muscle and serum was negatively associated with ribeye areas.In summary,the amino acid profiles in the muscle and serum of Jinhua pigs presented developmental changes,and arginine,valine,isoleucine and leucine contents were positively correlated with muscle growth.