基于广泛靶向代谢组学的武穴野生山药及其零余子代谢物差异分析

Comparative metabolite profiling of rhizome and bulbil of wild Chinese yam(Dioscorea polystachya Turcz.)from Wuxue by widely targeted metabolomics

为揭示武穴野生山药及其零余子中代谢物的种类与相对含量差异,利用LC-MS/MS广泛靶向代谢组学的方法对采集自湖北省武穴市野生山药的山药肉、山药皮和零余子中代谢物成分进行检测,并结合多元统计分析和单变量统计分析筛选零余子与山药肉间、山药皮与山药肉间的差异代谢物。结果表明:武穴野生山药及其零余子中共检测到385种代谢物;在零余子与山药肉间共筛选到70种差异代谢物,其中,43种代谢物在零余子中相对含量较高,27种代谢物在山药肉中相对含量较高。在山药皮与山药肉间共筛选出81种差异代谢物,其中,46种代谢物在山药皮中相对含量较高,35种代谢物在山药肉中相对含量较高。武穴野生山药的山药肉中富含缬氨酸、甲硫氨酸、丙氨酸等多种氨基酸,零余子中富含异柚葡糖苷、C-hexosyl-chrysoeriol 5-O-hexoside、扁蓄苷等18种黄酮类代谢物,山药皮中则富含反式玉米素-O-葡糖苷核苷、乙酰左旋肉碱、维生素B_(2)、C-hexosyl-chrysoeriol 7-O-hexoside、槲皮素-3-甲基醚等代谢物。综上,武穴野生山药的块茎及其零余子中含有丰富的代谢物,可根据代谢物种类、功能与用途合理开发应用。

To clarify the metabolites and their relative contents in rhizome and bulbil of wild Chinese Yam(Dioscorea polystachya Turcz.)from Wuxue,widely targeted LC-MS/MS was used to determine the metabolite profiles in rhizome flesh,rhizome peel,and bulbil of wild Chinese yam from Wuxue,Hubei Province.Differentially accumulated metabolites between bulbil and rhizome flesh and between rhizome peel and rhizome flesh were identified by multivariate and univariate analyses.The result showed that a total of 385 metabolites were detected in rhizome and bulbil of wild Chinese yam from Wuxue.There were 70 differentially accumulated metabolites between bulbil and rhizome flesh,among which 43 metabolites were more significantly accumulated in bulbil,while 27 metabolites had higher accumulation in rhizome flesh.Moreover,a total of 81 differentially accumulated metabolites were identified between rhizome peel and rhizome flesh,including 46 metabolites with higher accumulation in rhizome peel and 35 metabolites with higher accumulation in rhizome flesh.In addition,the rhizome flesh of wild Chinese yam was enriched in amino acids such as valine,methionine and alanine.Compared with rhizome flesh,bulbil had higher levels of 18 flavonoid metabolites such as isohemiphloin,C-hexosyl-chrysoeriol 5-O-hexoside and avicularin,while the rhizome peel was more enriched in trans-zeatin-O-glucoside riboside,acetyl-L-carnitine,vitamin B_(2),C-hexosyl-chrysoeriol 7-O-hexoside and quercetin-3-methyl ether.This study demonstrated that rhizome and bulbil of wild Chinese yam from Wuxue contain abundant metabolites,and could be rationally developed and utilized based on the type and function of metabolites.