优化顶空固相微萃取-气相色谱-质谱联用方法测定蜂蜜挥发性成分的研究

Optimization of Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry for the Determination of Volatile Components in Honey

为获得蜂蜜中挥发性成分的最佳检测条件,以总峰面积和峰数量为指标,采用顶空固相微萃取-气相色谱-质谱联用方法进行检测,通过设计单因素实验对挥发性成分的提取参数条件进行了优化。结果表明,最佳提取条件为蜂蜜称样量8 g,使用65 μm PDMS/DVB萃取头,在60 ℃下萃取60 min。

In order to obtain the best detection conditions for volatile components in honey,the total peak area and the number of peaks were used as indicators to detect the volatile components by headspace solid-phase microextraction-gas chromatography-mass spectrometry.The extraction parameter conditions were optimized.The results showed that the optimal extraction conditions were 8 g honey sample,65 μm PDMS/DVB extraction tip,and extraction at 60 ℃ for 60 min.