碳酸酐酶Ⅸ蛋白在鼻咽癌组织中的表达及其与预后的关系

Expression of carbonic anhydraseⅨprotein in nasopharyngeal carcinoma and its relationship with prognosis

目的探讨碳酸酐酶Ⅸ(CAⅨ)在鼻咽癌组织中的表达及与预后的关系。方法选取2013年1月至2015年1月泰州市人民医院保存的鼻咽癌组织标本122例,同时选取慢性鼻咽炎性组织标本60例作为对照组,采用免疫组化染色法检测CAⅨ蛋白表达,随访病人无进展生存期。结果鼻咽癌组CAⅨ蛋白阳性表达率为55.74%,明显高于慢性鼻咽炎性组的16.67%,差异有统计学意义(P<0.05);CAⅨ蛋白表达与淋巴结转移有密切关联(P<0.05),与病人年龄、性别、肿瘤长径、TNM分期、病理类型和关联性不大(P>0.05);CAⅨ蛋白阳性表达组中位无进展生存期为46个月(95%CI:31.92~48.08),明显低于CAⅨ蛋白阴性表达组,差异有统计学意义(P<0.05);COX回归分析结果显示,TNM分期、淋巴结转移和CAⅨ蛋白表达是鼻咽癌病人预后(无进展生存)的危险影响因素(HR=2.06、1.65和1.47,P<0.05)。结论鼻咽癌组织CAⅨ蛋白表达上调,与病人预后有关。

Objective To investigate the expression of carbonic anhydraseⅨ(CAⅨ)in nasopharyngeal carcinoma and its relation⁃ship with prognosis.Methods One hundred and twenty-two specimens of nasopharyngeal carcinomain Taizhou People's Hospital from January 2013 to January 2015 were selected,and 60 chronic nasopharyngeal inflammatory tissues were selected as control group,the expression of CAⅨprotein was detected by immunohistochemical staining,and the progression-free survival time of patients was fol⁃lowed up.Results The positive expression rate of CAⅨprotein in nasopharyngeal carcinoma group was 55.74%,which was signifi⁃cantly higher than that in chronic nasopharyngeal inflammatory group(16.67%)(P<0.05).The expression of CAⅨprotein was closely associated with lymph node metastasis(P<0.05),and not significantly correlated with age,sex,tumor diameter,TNM stage,pathological type and lymph node metastasis(P>0.05).The median progression-free survival of CAⅨprotein positive expression group was 46 months(95%CI:31.92-48.08),which was significantly lower than that of the CAⅨprotein expression negative group,the difference was statistically significant(P<0.05).COX regression analysis showed that TNM stage,lymph node metastasis and CAⅨprotein expression was the prognosis of patients with nasopharyngeal carcinoma(progression-free survival)risk factors(HR=2.06,1.65 and 1.47,P<0.05).Conclusion Nasopharyngeal carcinoma tissue CAⅨprotein expression level is up-regulated,and related to the prognosis of patients.