海参体腔液提取物对南美白对虾自溶的抑制作用

Inhibitory effect of sea cucumber coelomic fluid extracts on white shrimp autolysis

目的:研究海参加工副产物海参体腔液提取物对南美白对虾虾靡自溶中蛋白质降解的抑制作用。方法:通过聚丙烯酰胺凝胶电泳(SDS-PAGE)对南美白对虾自溶中蛋白质的降解情况进行分析,并进一步利用SDS-PAGE和三氯乙酸(TCA)可溶性寡肽释放量指标分析海参体腔液及其提取物对南美白对虾自溶中蛋白质降解的影响。结果:南美白对虾自溶的适宜条件为pH 3.0、孵育温度50℃、孵育时间2 h。在此条件下,南美白对虾的肌球蛋白重链(200 kDa左右)和肌动蛋白(44.3 kDa左右)的条带灰度均有不同程度的降低,表明蛋白质发生降解。海参体腔液对南美白对虾自溶中蛋白质降解起到一定抑制作用,当海参体腔液提取物添加量为6.25~25.00 g/100 g虾靡时,南美白对虾自溶中蛋白质降解被显著抑制;提取物添加量为12.50~25.00 g/100 g虾靡时,也显著抑制TCA可溶性寡肽的释放。结论:海参体腔液提取物能有效抑制南美白对虾蛋白质的降解,对虾靡自溶发挥一定抑制功效。

Objective:In this paper,the inhibition of protein degradation in autolysis of white shrimp(Penaeus vannamei)surimi by extracts from the coelomic fluid of sea cucumber(Stichopus japonicus),a by-product of sea cucumber processing,was studied.Methods:The protein degradation in autolysis of white shrimp was studied by SDS-PAGE assay.Furthermore,the effect of sea cucumber coelomic fluid and its extracts on white shrimp protein degradation was determined by SDS-PAGE and TCA-soluble oligopeptide assay.Results:The optimal conditions of white shrimp autolysis were incubation at pH 3.0,50℃for 2 h.The bond intensities of myosin heavy chain around(200 kDa)and actin around(44.3 kDa)decreased suggesting the protein degradation of white shrimp.The sea cucumber coelomic fluid showed inhibitory effect on protein degradation in autolysis of white shrimp.When the concentration of coelomic fluid extract of sea cucumber was in the range of 6.25%~25.00%,the protein degradation was significantly inhibited.The release of TCA-soluble oligopeptide was also significantly inhibited by coelomic fluid extract at 12.50%~25.00%.Conclusion:Sea cucumber coelomic fluid extracts possess inhibitory effect on protein degradation of white shrimp,indicating suppression of white shrimp surimi autolysis.