不同品种及产地鲜天麻挥发性物质差异性分析

Differential analysis of volatile substances in fresh Gastrodia elata from different varieties and origin

目的:分析不同品种和不同产地鲜天麻中挥发性化合物的差异。方法:采用气相—离子迁移色谱(GC-IMS)技术对6个产地、3个品种的鲜天麻挥发性物质成分进行测定,利用VOCal和Reporter、Gallery Plot和Dynamic PCA三款插件及“最近邻”指纹分析,分别从不同角度对样品进行定量定性分析。结果:研究共检出75种挥发性化合物,其中可以鉴定出的物质45种,包括16种醛类、9种酯类、6种醇类、5种酮类和3种酸类物质。采用主成分分析能够有效区分不同品种鲜天麻样品,但对不同产地鲜天麻区分有效性差。结论:GC-IMS可以实现对不同品种鲜天麻样品挥发性化合物的差异进行快速分析。

Objective:This study aimed to analyze the differences of volatile compounds among fresh Gastrodia elata of different varieties and origins.Methods:Volatile components of fresh G.elata from six origin and three varieties were analyzed by gas chromatography-ion mobility spectrometry(GC-IMS)technology,and quantified and qualitatively from different angles using VOCal and Reporter,Gallery Plot and Dynamic PCA inserts and"nearest neighbor"fingerprint analysis.Results:In this study,a total of 75 kinds of volatile compounds were detected.Among them,45 kinds of compounds were identified,including 16 aldehydes,9 esters,6 alcohols,5 ketones and 3 acids.Principal component analysis(PCA)was used to effectively distinguish different varieties of fresh G.elata.However,it was not sensitive enough to distinguish the G.elata from different origins.Conclusion:GC-IMS can be used to quickly analyze the differences of volatile compounds in different varieties of fresh G.elata.