胶质瘤中叉头框蛋白N2与F2的表达及意义

Expression and significance of fork head box protein N2 and F2 in gliomas

目的 观察叉头框蛋白N2(FOXN2)与叉头框蛋白F2(FOXF2)在正常脑组织和各级别胶质瘤中的表达情况,探讨两者与胶质瘤级别的关系,推测其在胶质瘤发生发展中的作用,为胶质瘤的治疗寻找可能的药物治疗靶点。方法 从皖南医学院弋矶山医院病理科调取36例2016年1月~2020年12月脑胶质瘤患者手术切除的胶质瘤组织标本,双盲法进行免疫组织化学和免疫荧光染色检测FOXN2、FOXF2的表达情况;采用SPSS 18.0统计学软件进行分析,以P<0.05为差异有统计学意义。再对2020年1月~2021年1月皖南医学院弋矶山医院神经外科手术中获取的新鲜正常脑组织、低级别胶质瘤组织和高级别胶质瘤组织进行Western blotting、Real-time PCR实验(正常脑组织均来自外伤手术患者)。结果 免疫组织化学与免疫荧光结果表明,胶质瘤级别越高FOXN2与FOXF2的表达越低(P<0.05);Western blotting实验显示,正常脑组织及低级别胶质瘤中FOXN2和FOXF2表达都高于高级别胶质瘤组(P<0.05);Real-time PCR结果显示,正常脑组织中FOXN2和FOXF2的表达同样高于高级别胶质瘤组(P<0.01)。结论 FOXN2和FOXF2在正常脑组织中有表达,在胶质瘤中低表达且呈级别依赖性,提示FOXN2和FOXF2与胶质瘤的级别及不良预后有关,增强FOXN2和FOXF2的表达有望成为胶质瘤治疗的靶点。

Objective To observe the expression levels of fork head box protein N2(FOXN2) and fork head box protein F2(FOXF2) in normal brain tissue, low-grade glioma and high-grade glioma, and to explore the relationship between the two and the grade of glioma, and then deduce their roles in the occurrence and development of glioma, to look for possibilities target of drug therapy. Methods From January 2016 to December 2020, 36 glioma specimens were collected from the Department of Pathology, Yijishan Hospital, Wannan Medical College. The expressions of FOXN2 and FOXF2 were detected by immunohistochemistry and immunofluorescence with the double-blind method, and analyzed by SPSS18.0 software, with a statistical significance of P<0.05. Then Western blotting and Real-time PCR experiments were carried out on fresh normal brain tissues, low-grade glioma tissues, high-grade glioma tissues during neurosurgery in Yijishan Hospital of Wannan Medical College from January 2020 to January 2021(normal brain tissues were all trauma patients). Results The results of immunohistochemistry and immunofluorescence showed that the higher grade of glioma, the lower expressions of FOXN2 and FOXF2 would be(P<0.05). Western blotting showed that the expressions of FOXN2 and FOXF2 in normal brain tissue and low-grade glioma were higher than those in high-grade glioma(P<0.05). The result of Real-time PCR showed that the expressions of FOXN2 and FOXF2 in normal brain tissue were higher than those in high-grade glioma(P<0.01). Conclusion FOXN2 and FOXF2 are expressed in normal brain tissues, and their expression is low in glioma in a grade-dependent manner, suggesting that FOXN2 and FOXF2 are related to the grade and poor prognosis of glioma. Enhancing the expression of FOXN2 and FOXF2 may provide a new idea for target therapy of glioma.