喙花姜块茎和根中挥发油成分分析及其α-葡萄糖苷酶抑制活性

Analysis of Volatile Oils from Tubers and Roots of Rhynchanthus beesianus and Their α-Glucosidase Inhibitory Activities

通过气相色谱-质谱联用技术分析鉴定水蒸气蒸馏法提取的喙花姜块茎和根中挥发油的化学成分,再对其主要化合物进行检索分析其安全性。通过4-硝基苯基-β-D-吡喃半乳糖苷(4-nitrophenyl-β-D-galactoside,PNPG)法分析其α-葡萄糖苷酶抑制活性。结果表明,喙花姜块茎和根中挥发油的化学成分大致相似,但仍有部分差异,主要成分均为单萜和倍半萜类,其中桉油精相对含量最高,分别达到48.81%和37.23%。其主要化学成分也具有较好的食用安全性。喙花姜块茎和根中挥发油α-葡萄糖苷酶的抑制活性高于阳性对照阿卡波糖。

The chemical components of volatile oil from the tubers and roots of Rhynchanthus beesianus extracted by steam distillation were analyzed and identified by gas chromatography-mass spectrometry. The main compounds were evaluated concerning their safety. An assay using 4-nitrophenyl-β-D-galactoside as substrate analyzed α-glucosidase inhibitory activity. The chemical components of the volatile oil in the tuber and root of R. beesianus were similar,but some differences were evident. The main components were monoterpenes and sesquiterpenes. The relative content of Eucalyptus was the highest,reaching 48.81% and 37.23% respectively.The main chemical components were safe to consume. Effects of volatile oil from tubers and roots of R. beesianus on α-glucosidase inhibitory activity were higher than the acarbose positive control.