玉米糖肽的纳滤脱盐工艺

Desalination of Zein Glycopeptides via Nanofiltration Membrane

为了去除玉米糖肽溶液中过多的盐分,采用相对分子质量200纳滤膜对具有抗氧化活性的玉米糖肽溶液进行脱盐处理,通过对膜通量以及玉米糖肽溶液的Na;质量分数、脱盐率、电导率、短肽回收率和纳滤后产物自由基清除率和Fe;-螯合率等参数的研究,确定玉米糖肽溶液的纳滤脱盐工艺。结果表明,利用相对分子质量200纳滤膜对玉米糖肽溶液脱盐的工艺条件为:在压力2.0 MPa、温度20℃条件下连续纳滤5次,每次体积浓缩倍数为2。经5次纳滤后,玉米糖肽截留液的电导率降低60.87%,脱盐率和短肽回收率分别为81.60%和96.97%,羟自由基清除率从11.25%提高到15.85%,而1,1-二苯基-2-三硝基苯肼自由基清除率和Fe;-螯合率分别降低11.83%和46.07%。同时,相对分子质量200纳滤膜还可以除去糖基化反应体系中55.54%未反应的D-氨基葡萄糖,起到脱盐、脱糖和浓缩的三重作用。

In order to remove the excessive salt from zein glycopeptide solution,the nanofiltration membrane with a molecular weight cut-off of 200 was applied for the desalination of antioxidant zein glycopeptide solution.The membrane flux,Na;mass fraction,desalination rate,electrical conductivity,recovery rate of short peptide of zein glycopeptide solution,and the free radical scavenging rate and Fe;-chelating activity of products after nanofiltration were studied to determine the nanofiltration desalination process of zein glycopeptides.The results showed that zein glycopeptides was desalted by a 200 nanofiltration membrane was carried out continuously for 5 times nanofiltration under 2.0 MPa at 20℃ with each nanofiltration volumetric concentration factor of 2.After 5 times of nanofiltrations,the electrical conductivity of retentate was decreased by60.87%.The desalting rate and the recovery rate of short zein glycopeptide were 81.60%and 96.97%,respectively.The hydroxyl radical scavenging rate of desalted zein glycopeptide was increased from 11.25%to 15.85%,and the 1,1-diphenyl-2-picrylhydrazyl radical scavenging rate and Fe;-chelating activity were decreased by 11.83% and 46.07%,respectively.Moreover,the 200 nanofiltration membrane could remove 55.54%of unreacted D-glucosamine in the glycosylation reaction product,playing a triple role of desalination,desugarization and concentration.