摘要
目的:探讨紫苏粗提物体外对酶抑制剂的作用,初步阐明其对代谢综合征的药效物质基础。方法:测定紫苏粗提物中黄酮及酚酸含量,探明与酶抑制有关的活性成分。通过体外实验,以抑制率为指标,测定紫苏粗提物对不同酶活性的抑制作用,以IC_(50)为指标评价抑制活性。结果:紫苏粗提物中黄酮含量0.3098(mg/mg),总酚酸含量58.59(mg/g)。紫苏对α-淀粉酶抑制的IC_(50)值为0.273(mg/mL),对黄嘌呤氧化酶的IC_(50)值为0.244(mg/mL),对胰脂肪酶的IC_(50)值为0.347(mg/mL),对乙酰胆碱酯酶的IC_(50)值为0.018(mg/mL)。结论:紫苏粗提取对四种酶都有一定的抑制活性,且呈现剂量-效应关系。初步阐明其治疗代谢综合征的药效物质基础为黄酮。可将紫苏中黄酮类化合物进一步开发利用。
Objective:The purpose of the research is to explore the effect of enzyme inhibitors of active substances in crude extracts of Perilla frutescens L.Britt.,and to preliminarily elucidate it’ s pharmacological substance basis on hyperglycemia,hyperuric acid and hyperlipidemia.Methods:The content of flavonoids and phenolic acid in UV extract was determined and showed the active components associated with enzyme inhibition.In vitro experiments,the inhibition of basilla extract on different enzyme activity was determined and the inhibitory activity was evaluated by IC_(50).Results:Flavone content in the crude UV extract was 0.3098 (mg/mg)and the total phenolic acid content was 58.59 (mg/g).Perilla had a IC_(50)for α-amylase inhibition of 0.273 (mg/mL),0.244 (mg/mL)for xanthine oxidase,0.347 (mg/mL)for pancreatic lipase,and 0.018 (mg/mL)for acetylcholinestase.Conclusion:The perilla crude extracts all have certain inhibitory activities on four enzymes,and presents a dose-effect relationship.It is preliminarily elucidated that flavonoids are the basis of pharmacodynamic substances in the treatment of metabolic syndrome.Flavonoid compound in purilla can be further developed and utilized.
作者
余雨婷
张彦
张迎
李娜娜
郑梦迪
闫平
柴希艳
同红娟
YU Yuting;ZHANG Yanl;ZHANG Yingl;LI Nana;ZHENG Mengdi;YAN Ping;CHAI Xiyan;TONG Hongjuan(Xi’an Medical University,Xi’an 710021;Xi’an Yurun Baide Healthy Management Co.,Ltd.,Xi’an 710061;Xi’an Quality Life Agricultual Technology Development Industrial Co.,Ltd.,Xi’an 710065)
出处
《中国食品添加剂》
CAS
北大核心
2022年第4期63-71,共9页
China Food Additives
基金
西安医学院2021年大学生创新创业训练项目121521054
西安医学院2021年省级大学生创新创业训练项目计划项目121521054
陕西省科协提升公共素质项目2021PSL43
陕西省榆林市科技局CXY-2020-092:陕北黑豆抗氧化功能保健品开发
陕西省科技厅一般青年项目2021JQ-782紫苏品种鉴别和品质评价研究。
关键词
代谢综合征
紫苏
黄酮
总酚酸
酶抑制剂
metabolic syndrome
Perilla frutescens L.Britt.
flavonoids
the total phenolic acid
enzymatic inhibitors