加压毛细管电色谱法测定葛根类食品中9种异黄酮

Determination of nine isoflavones in pueraria foods by pressurized capillary electrochromatography

建立加压毛细管电色谱法检测葛根类食品中9种异黄酮含量的分析方法,并依据所建立的方法研究不同含葛根食品中异黄酮含量情况。样品由20%乙醇溶液(含0.1%甲酸)提取,经PRiMEHLB小柱净化后检测。以乙腈-15mmol/L磷酸钾缓冲液(15∶85,V∶V)为流动相,在电压强度+2kV条件下,外标法峰面积定量。9种异黄酮标准溶液在10.0~200.0μg/mL浓度范围内线性良好,检出限范围为0.5~1.0mg/kg,定量限范围为2.0~5.0mg/kg,加标回收率达到86.2%~98.6%。该方法具有前处理简单、净化效果好、灵敏度高的优点,适用于含葛根复杂基质类食品中多种异黄酮的分离和定量检测。

To establish an analytical method for the determination of nine isoflavones in pueraria foods by pressurized capillary electrochromatography,and to study the changes of isoflavones under different pueraria foods according to the established method.The sample was extracted by 20% ethanol solution(containing 0.1% formic acid)and purified by PRiME HLB column.Under the condition of voltage strength +2kV,the acetonitrile-15mmol/L potassium phosphate buffer(15∶85,V∶V)was used as the mobile phase and the peak area was quantified by external standard method.The linearity of the nine isoflavones standard solution was good within the concentration range of 10.0~200.0μg/mL.The limits of detection of 0.5~1.0mg/kg,and the limits of quantitation of 2.0~5.0mg/kg.The recoveries were up to 86.2%~98.6%.The method has the advantages of simple pretreatment,good purifying effect and high sensitivity.It was suitable for the separation and quantitative determination of a variety of isoflavones in complex matrix foods containing pueraria foods.