褐藻多糖的体外抗氧化活性及其延长秀丽隐杆线虫的寿命

In Vitro Antioxidant Activity of Fucoidan-containing Sulfated Polysaccharide and Its Prolongation of the Lifespan of Caenorhabditis elegans

该研究探讨了褐藻多糖(FSP)的体外抗氧化作用。以秀丽隐杆线虫为模式动物,采用氧化剂H2O2建立急性氧化应激模型,测定FSP对线虫寿命、运动能力、年龄色素及氧化应激指标的影响。研究表明,FSP对羟基自由基有一定的清除能力,且作用效果呈剂量依赖性;FSP(50、100、200μg/mL)呈剂量依赖性的延长秀丽隐杆线虫寿命,分别为7.24%、26.70%和48.42%;FSP显著提高了线虫运动能力,其中200μg/mL处理组运动能力达73.50%,而且减少体内脂褐素的积累及细胞凋亡程度;在0.1%H2O2氧化应激下,200μg/mL的FSP通过提升抗氧化酶SOD、CAT活性来增强抗氧化能力;qRT-PCR结果显示,FSP通过调控Insulin/IGF-1信号通路上相关基因的表达影响线虫寿命。综上,FSP可通过延长秀丽隐杆线虫的寿命,提升其运动能力,提升线虫抗氧化酶活性,调控关键基因的表达,抑制氧化应激水平,对线虫氧化损伤发挥保护作用。

The in vitro antioxidant effect of fucoidan-containing polysaccharide(FSP)was investigated.Taking Caenorhabditis elegans(C.elegans)as the model animal,an acute oxidative stress model was established using the oxidizing agent,H2O2.The effects of FSP on lifespan,mobility,age pigment and oxidative stress indices were determined.The research showed that FSP exhibited an ability to scavenge hydroxyl radicals and this effect was in dose-dependent;FSP(50,100 and 200μg/mL)prolonged the lifespan of C.elegans in a dose-dependent manner(by 7.24%,26.70%and 48.42%,respectively);FSP significantly increased the motility of nematodes,with the motility of the 200μg/mL FSP treatment group reaching 73.50%,and the accumulation of lipofuscin and the degree of apoptosis decreased;Under 0.1%H2O2-induced oxidative stress,the treatment with 200μg/mL FSP enhanced the antioxidant capacity through increasing the activities of antioxidant enzymes,SOD and CAT;The qRT-PCR results showed that FSP influenced the lifespan of nematodes through regulating the expression of related genes in the Insulin/IGF-1 signaling pathway.In summary,FSP could exert protective effects on oxidative damage in nematodes through extending the lifespan of C.elegans,enhancing its mobility,promoting the activities of antioxidant enzymes,regulating the expression of key genes and inhibiting the level of oxidative stress.