制药废水中青霉素与厌氧污泥的相互作用机制

Interaction mechanism between penicillin and anaerobic sludge in pharmaceutical wastewater

通过批式实验考察了制药废水中青霉素G钠盐与厌氧污泥的相互作用机制。结果表明:青霉素G钠盐的水(降)解反应符合伪一级动力学,在纯水、灭活和活性厌氧污泥条件下的速率常数分别为0.048、0.1216和0.1283 h^(-1)。灭活厌氧性污泥因其絮体表面的亲核官能团显著提高了青霉素G钠盐的水(降)解速率,活性厌氧污泥因其生物活性进一步提高了青霉素G钠盐的水(降)解速率。低浓度(≤10 mg·L^(-1))、中浓度(100 mg·L^(-1))和高浓度(1000 mg·L^(-1))的青霉素G钠盐对厌氧污泥的产甲烷活性分别呈现无显著抑制作用、有一定的抑制作用、有显著抑制作用。投加中、高浓度青霉素G钠盐的厌氧污泥的甲烷产量分别是正常厌氧污泥的64.0%和19.9%。中高浓度青霉素G钠盐主要直接抑制水解酸化菌,从而间接影响产甲烷活性。

Batch tests were conducted to investigate the interaction mechanism between penicillin G sodium and anaerobic sludge for pharmaceutical wastewater treatment.The results showed that hydrolysis(degradation)of penicillin G sodium followed pseudo-first-order reaction kinetics,and the rate constants were 0.048 h^(-1),0.1216 h^(-1) and 0.1283 h^(-1) in pure water,inactivated and activated anaerobic sludge,respectively.Inactivated anaerobic sludge significantly increased the hydrolysis(degradation)rate of penicillin G sodium due to the nucleophilic function groups on its floc surface,while activated anaerobic sludge further increased the hydrolysis(degradation)rate of penicillin G sodium due to its biological activity.Low-concentration(≤10 mg·L^(-1)),medium-concentration(100 mg·L1-1)and high-concentration(1000 mg·L-)penicillin G sodium had no significant inhibition effect,some inhibition effect and significant inhibition effect on the methanogenic activity of anaerobic sludge,respectively.The methane yields of anaerobic sludge with addition of mediumconcentration(100 mg·L^(-1))and high-concentration(1000 mg·L^(-1))penicillin G sodium were 64.0%and 19.9%of normal anaerobic sludge without penicillin G sodium addition,respectively.Penicillin G sodium with medium-high concentration(100~1000 mg·L^(-1))mainly inhibited acidogenic bacteria directly and thus affected methanogenic activity indirectly.