复褥方对大鼠皮肤刺激性和压疮创面局部病理改变的观察

Observation on Local Pathological Changes of Skin Irritation and Pressure Ulcer Wounds in Rats by Compound Decubitus Prescription

目的对复褥方进行大鼠皮肤刺激性试验并对大鼠压疮创面的局部病理改变进行观察,评估其安全性。方法(1)将大鼠随机分为完整皮肤组和破损皮肤组,分别进行一次和多次给药的皮肤刺激性实验,观察去除药物后大鼠的皮肤反应情况。(2)将大鼠随机分为4组,即正常组(A组)、模型组(B组)、紫莲膏组(C组)、复褥方组(D组),除正常组外,其余3组制作大鼠压疮模型。造模成功后,在常规处理的基础上,C组创面再涂紫莲膏,D组创面再涂复褥方。每天2次,连续10 d。分别在给药后第0、3、7、10 d后测量创面面积。末次给药后处死大鼠,取创面组织进行HE染色。结果(1)复褥方一次给药对完整皮肤组无刺激,均未出现红斑、水肿等现象,对破损皮肤组去药1 h后有轻微刺激,表现为皮肤的轻微水肿,可自行消退,未再出现损伤现象。复褥方多次给药对完整皮肤组、破损皮肤组均无刺激等现象。(2)与B组相比较,C组、D组创面面积均显著缩小(P<0.01);C组与D组比较,D组缩小面积优于C组(P<0.05);C组和D组HE染色结果显示炎性浸润程度明显减轻,新生肉芽组织明显增多;C组与D组比较,D组炎性浸润明显减轻,新生肉芽组织明显增多。结论(1)复褥方单次使用对大鼠完整皮肤无刺激,对破损皮肤有轻微刺激,24h可自行消退;多次使用对大鼠完整皮肤、破损皮肤均无刺激,有较好的安全性。(2)复褥方具有促进大鼠压疮创面愈合和抗炎的作用,这可能是复褥方治疗大鼠压疮的机制之一。

Objective:To make a skin irritation test of Compound Decubitus Prescription in rats and observe the local pathological changes of the pressure ulcer wounds in rats,and to evaluate its safety and efficacy.Methods:(1)The rats were randomly divided into an intact skin group and a damaged skin group.Skin irritation experiments were carried out for one and multiple administration respectively,and the skin reaction of the rats after removing the drug was observed.(2)The rats were randomly divided into 4 groups,namely,normal group(group A),model group(group B),purple lotus paste group(group C),and Compound Decubitus Prescription group(group D).The other three groups were used to make pressure ulcer models in rats.After successful modeling and on the basis of routine treatment,the wounds of group C were re-applied with purple lotus paste,and the wounds of group D were re-applied with Compound Decubitus Prescription,twice a day for 10 consecutive days.The wound area was measured at the 0,3rd,7th and 10th days after administration respectively.The rats were sacrificed after the last administration and their wound tissue was collected for HE staining.Results:(1)The Compound Decubitus Prescription group did not irritate the intact skin at one-time administration and no erythema and edema occurred.In the damaged skin group,there was slight irritation one hour after the drug was removed.No further damage occurred.The repeated administration of Compound Decubitus Prescription did not cause irritation to the intact skin group and the damaged skin group.(2)Compared with that of group B,the wound area of group C and group D was significantly reduced(P<0.01);compared between group C and group D,the reduced area of group D was better than that of group C(P<0.05).The results of HE staining in group C and group D showed that the degree of inflammatory infiltration was significantly reduced,and the new granulation tissue was significantly increased;compared with that in group C,the inflammatory infiltration in group D was significantly reduced,and the new granulation tissue was significantly increased.Conclusion:(1)The one-time use of Compound Decubitus Prescription has no irritation to the intact skin of rats,but has a slight irritation to the damaged skin,and it can subside on its own within 24 hours.The repeated use has no irritation to the intact skin and damaged skin of rats,with good effect and safety.(2)Compound Decubitus Prescription has the effect of promoting wound healing and anti-inflammatory effect of pressure ulcers in rats,which may be one of the mechanisms of Compound Decubitus Prescription in treating pressure ulcers in rats.