刺角瓜提取物对南方根结线虫的触杀活性研究

The Contact Activity of Extracts from Cucumis metuliferus against Meloidogyne incognita

采用触杀法研究了刺角瓜无水乙醇提取物对南方根结线虫2龄幼虫的毒杀活性。结果表明,不同浓度的刺角瓜无水乙醇根和茎叶提取物对南方根结线虫有不同程度的毒杀活性,其中,用1.00 mg/mL的刺角瓜根、茎叶乙醇提取物分别处理,24 h后南方根结线虫校正死亡率分别为98.95%、94.46%,48 h后南方根结线虫校正死亡率分别为100%、98.12%,与1.00 mg/mL阿维菌素的活性无明显差异。毒力测定结果表明,茎叶提取物处理24和48 h,对南方根结线虫的半致死浓度LD_(50)分别为0.7032和0.4503 mg/mL;根提取物处理24和48 h,对南方根结线虫的半致死浓度LD_(50)分别为0.4935和0.4842 mg/mL。此外,根提取物和茎叶提取物的杀线虫活性表现出明显差异。

The contact toxicity of ethanol extract from Cucumis metuliferus against the 2nd instar larvae(J2)of Meloidogyne incognita was studied.The results showed that different concentrations of ethanol extracts from roots and stems and leaves of Cucumis metuliferus had different degrees of toxic activity against Meloidogyne incognita.The corrected mortality rates of Meloidogyne incognita were 98.95%and 94.46%24 h after treatment with 1.00 mg/mL ethanol extracts from roots and stems and leaves of Cucumis metuliferus,and 100%and 98.12%48 h after treatment,respectively,which were not significantly different from the activity of 1.00 mg/mL abamectin.The results of toxicity test showed that the LD_(50) of stem and leaf extracts to Meloidogyne incognita were 0.7032 and 0.4503 mg/mL respectively after 24 and 48 h treatment.After 24 and 48 h of root extract treatment,the LD_(50) of root extract against Meloidogyne incognita was 0.4935 and 0.4842 mg/mL,respectively.Nematicidal activity of root extract and stem and leaf extract showed significant difference.