毛竹内生拮抗细菌对食用菌病害的生防机理研究

The Biological Control Mechanism of Endophytic Antagonistic Bacteria in Phyllostachys edulis on Edible Fungi Diseases

研究了毛竹内生拮抗细菌JL-B05和JL-B16对3种食用菌病原菌的生防机理。结果表明,不同浓度内生拮抗细菌过滤液和灭菌液对病原菌菌丝生长均具有显著的抑制效果,其中浓度为20%的内生拮抗细菌JL-B05过滤液对病原菌菌丝生长的抑制效果最好,对疣孢病菌的抑制率达86.37%,对油疤病菌的抑制率为78.81%,对蛛网病菌的抑制率为76.76%,抑制效果均优于内生拮抗细菌JL-B16过滤液。浓度为20%的内生拮抗细菌JL-B16灭菌液对油疤病菌菌丝生长的抑制率为72.11%,对疣孢病菌抑制率为62.51%,对蛛网病菌的抑制率为61.88%,抑制效果均优于内生拮抗细菌JL-B05灭菌液。在内生拮抗细菌发酵液和灭菌液对病原菌孢子萌发抑制效果测定中,浓度为40%内生拮抗细菌JL-B05、JL-B16过滤液和灭菌液对3个病原菌的抑制效果均较为显著。内生细菌JL-B05、JL-B16挥发性物质对病原菌菌丝生长均表现出一定的抑制作用。

The biological control mechanism of endophytic antagonistic bacteria JL-B05 and JL-B16 against three edible fungus pathogens was studied.The results showed that different concentrations of endophytic antagonistic bacterial filtration solution and sterilized solution had significant inhibitory effect on the growth of pathogenic mycelia,among which the 20%endophytic antagonistic bacteria JL-B05 filtration solution had the best inhibitory effect on the growth of pathogenic mycelia,the inhibition rate of Mycogone perniciosa was 86.37%,the inhibition rate of Scytalidium lignicola was 78.81%,the inhibition rate of Cladobotryum semicirculare was 76.76%,and the overall inhibition effect was better than that of endophyte JL-B16 filtration solution.The inhibition rate of endophytic antagonistic bacteria JL-B16 sterilized solution with concentration of 20%on mycelial growth of Scytalidium lignicola was 72.11%,62.51%on Mycogone perniciosa and 61.88%on Cladobotryum semicirculare,and the overall effect was better than that of endophytic antagonistic bacteria JL-B05 sterilized solution.In the determination of the inhibitory effect of endophytic antagonistic bacterial filtration solution and sterilized solution on the spore germination of pathogenic bacteria,the concentration of 40%endophytic antagonistic bacteria JL-B05,JL-B16 filter solution and sterilized solution on the three pathogens was more significant.Endophytic bacteria JL-B05,JL-B16 volatile substances also showed a certain inhibition on the growth of pathogenic mycelia.