柚子幼果精油的提取及其抗氧化活性研究

Study on extraction and antioxidant activity of essential oil from grapefruit fruitlet

以柚子幼果为原料,采用亚临界流体萃取技术提取其精油,利用GC-MS联用技术对精油的化学成分进行分析,并应用AAPH诱导红细胞氧化应激模型对精油的抗氧化活性进行初步研究。结果表明:柚子幼果精油中共鉴定出126种有效成分,主要包括醇类、萜烯类、酮类、酯类、醛类、酚类和烷烃类7类化合物,其中醇类为主要化合物,共11种,相对含量为16.43%,萜烯类共10种,相对含量为5.37%,酮类共10种,相对含量为3.71%;经柚子幼果精油预处理后,AAPH诱导的红细胞溶血受到抑制,细胞外的MDA浓度和LDH释放量均减少,在保护细胞膜完整性的同时,也缓解了细胞的氧化应激反应,即柚子幼果精油能够通过清除自由基抑制AAPH引起的红细胞溶血。

Subcritical fluid extraction was used to extract the essential oil from grapefruit fruitlet,The chemical composition of the essential oil was analyzed by GC-MS,the antioxidant activity of the essential oil was preliminally investigated by using AAPH induced hemolysis model.The results showed that a total of 126 active components were identified in the essential oil of grapefruit fruitlet,including 7 main compounds:alcohols,terpenes,ketones,esters,aldehydes,phenols and alkanes.Alcohols were the main compounds,with a total of 11 species and a relative content of 16.43%.There were 10 terpenes with a relative content of 5.37%,and 10 ketones,with a relative content of 3.71%.After grapefruit fruitlet essential oil pretreatment,the erythrocyte hemolysis induced by AAPH was restrained,MDA concentration and LDH release quantity in extracellular decreased,which not only protected the cell membrane integrity,but also reduced oxidative stress reaction in the cell.It could be concluded that grapefruit fruitlet essential oil was able to remove free radicals,thereby inhibiting AAPH cause erythrocyte hemolysis.