Detachment-Independent Cationic-Dipeptide Beacons:Reduced False-Negative Signal and Accelerated Fluorescent Lighting in Cell Imaging

Nanocarriers have been developed to deliver DNA probes into cells for imaging analysis and improving their nuclease resist-ance.However,we found obvious lowered sensitivity and even false-negative results in the traditional DNA detachment-dependent fluorescent lighting mechanism.Here,we developed a detachment-independent fluorescent lighting mechanism integrated with cationic dipeptide nanoparticles(CDNs).CDNs displayed little quenching effect on fluorophores labeled on probes and improved the nuclease resistance of probes.In contrast to the traditional beacon,the fluorescence lighting was significantly accelerated without the need of desorption of the recognition products from the nanosurface,avoiding false-negative results from non-specific conformational adsorption.This work will open up new thoughts to improve the sensing performance of DNA probes in cell imaging,such as speed and sensitivity.