摘要
A label-free"light-on"colorimetric assay for ascorbic acid(AA)determination is unprecedentedly devised by regulating the intrinsic peroxidase-mimetic activity of the Fe-MIL-88.AA can reduce Fe(Ⅲ)presented in Fe-MIL-88 to Fe(Ⅱ),which will dramatically promote the hydroxyl radicals(·OH)conversion efficiency of H_(2)O_(2)through a Fenton reaction,and effectually accelerate the Fe-MIL-88 catalyzed chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine(TMB)-H_(2)O_(2).Redox reaction between AA and H_(2)O_(2)also can produce·OH and promote this chromogenic reaction.Thus,quantification of AA is real-ized via determining the enhanced colorimetric signals.The linear range is 0.5−40μM with a detection limit of 0.12μM.Furthermore,the straightforward yet effective colorimetric strategy with the merits of simplicity,cost-effectiveness,high accuracy,specificity,and sensitivity is widely utilized to determine the concentrations of AA in serological tests.
基金
supported by the National Natural Science Foundation of China(21904048,21902061 and 21902062)
the Natural Science Foundation of Shandong Province(ZR2019YQ10 and ZR2020QB033)
the Young Taishan Scholars Program(tsqn201812080).