不同基因型猕猴桃不同组织部位间AsA含量差异分析

Difference Analysis of AsA Content in Different Tissues of Different Genotypes Kiwifruit (Actinidia spp.)

【目的】猕猴桃果实营养丰富,以富含抗坏血酸(AsA)而著称,AsA是一种多功能的代谢物,与植物的生长发育密切相关,并且在植物组织内广泛存在。猕猴桃种类繁多,不同基因型猕猴桃间AsA含量存在较大差异,但不同基因型猕猴桃相同组织部位间及相同基因型猕猴桃不同组织部位间AsA含量差异分析少有报道,探明不同基因型猕猴桃不同组织部位间AsA含量富集差异,可为猕猴桃AsA代谢机制研究奠定理论基础。【方法】以中华猕猴桃红肉型‘红阳’、黄肉型‘金艳’,美味猕猴桃‘金魁’,毛花猕猴桃‘赣猕6号’,软枣猕猴桃‘魁绿’为试验材料,采用钼蓝比色法对幼叶、成叶、未木质化枝、木质化枝及果实的AsA含量进行测定。【结果】在中华猕猴桃、美味猕猴桃及软枣猕猴桃上均表现为幼嫩组织AsA含量低于成熟组织,而在毛花猕猴桃上表现为相反的趋势,且在幼嫩组织中均表现为毛花猕猴桃AsA含量显著性高于其它基因型猕猴桃,其中毛花猕猴桃‘赣猕6号’未木质化枝中AsA含量约是美味猕猴桃‘金魁’的10倍。此外,果实上任何组织部位都表现为毛花猕猴桃AsA含量显著性高于其它基因型,分别是中华猕猴桃红肉型‘红阳’的6.84~15.16倍,中华猕猴桃黄肉型‘金艳’的7.55~42.26倍,美味猕猴桃‘金魁’的11.49~21.12倍,软枣猕猴桃‘魁绿’的18.86~49.77倍。中华猕猴桃‘金艳’、美味猕猴桃‘金魁’及毛花猕猴桃‘赣猕6号’AsA含量由高到低均表现为果肉、果皮、果心。【结论】AsA在不同基因型猕猴桃不同组织器官中均有富集,其中中华猕猴桃、美味猕猴桃及软枣猕猴桃主要在成熟组织,尤其在成叶中富集,而毛花猕猴桃主要在果实中富集且显著性高于其它基因型猕猴桃。

[Objective]Kiwifruit is rich in nutrients,especially ascorbic acid(AsA).AsA is a multifunctional metabolite,which is closely related to the growth and development of plants and widely exists in plant tissues.AsA content varies in different genotypes of kiwifruit.However,there are few reports on the difference in AsA content in the same tissue part between different genotypes of kiwifruit and that in different tissue parts of the same genotype.Determining the difference of AsA content in various tissues of different genotypes kiwifruit would lay a theoretical foundation for future research on the metabolism mechanism of AsA.[Methods]The Actinidia chinensis red-flesh type ’Hongyang’ and A. chinensis yellow-flesh type ’Jinyan’,A. deliciosa ’Jinkui’,A. eriantha ’Ganmi 6’,A. arguta ’Kuilv’ were selected as test materials.The AsA content in young leaves,matures leaves,non-lingnified branch,lignified brache and fruits was determined by the molybdenum blue colorimetric method.[Results]The results showed that the AsA content of young tissues was lower than that of mature tissues in A. chinensis,A. deliciosa and A. arguta.However,the opposite trend was found in A. eriantha,and the AsA content of A. eriantha in young tissues was significantly higher than that of other genotypes of kiwifruit.The content of AsA in the non-lignified branches of A. eriantha ’Ganmi 6’ was approximately 10 times higher than that of A. arguta ’Kuilv’.In addition,the AsA content in any tissue of A. eriantha was significantly higher than that of other genotypes,which was 6.84-15.16 times higher than that of the A. chinensis red-flesh type ’Hongyang’,7.55 to 42.26 times that of the A. chinensis yellow-flesh type’Jinyan’,11.49-21.12 times that of the A.deliciosa ’Jinkui’,and 18.86-49.77 times that of the A. arguta’Kuilv’.Moreover,the hierarchy of AsA content in A. chinensis ’Jinyan’,A.deliciosa ’Jinkui’ and A. eriantha ’Ganmi 6’ was flesh > skin >core.[Conclusion]AsA was rich in all tissues and organs of different genotypes kiwifruit.Among them,AsA was mainly rich in mature tissues,especially mature leaves on A. chinensis red-flesh type’Hongyang’and A. chinensis yellow-flesh type ’Jinyan’,A. deliciosa ’Jinkui’ and A. arguta ’Kuilv’;while in A. eriantha ’Ganmi 6’,AsA was mainly rich in fruit and significance higher than other genotypes of kiwifruit.