DLX3和Syncytin-1 mRNA在妊娠期糖尿病患者胎盘钙化组织中的表达及意义

Expressions of DLX3 and Syncytin-1 mRNA in placental calcified tissues of patients with gestational diabetes mellitus and their significances

目的探讨妊娠期糖尿病患者(GDM)胎盘钙化组织中同源盒转录因子3(DLX3)和Syncytin-1 mRNA的表达及意义。方法选择2015年9月至2018年9月在广西壮族自治区人民医院产科收治的GDM患者30例作为观察组,另选择同期健康孕产妇30名作为对照组。比较两组的一般临床资料及胎盘钙化组织中DLX3和Syncytin-1 mRNA的表达水平(采用实时荧光定量聚合酶链式反应检测),探讨GDM发生的影响因素。结果观察组孕期体重增加量为(11.15±4.03)kg,对照组为(13.23±2.98)kg,两组比较差异有统计学意义(t=2.069,P=0.044)。观察组出现巨大儿3例(10.00%),对照组1例(3.33%),两组比较差异无统计学意义(χ^(2)=0.268,P=0.605)。观察组DLX3 mRNA表达水平显著高于对照组(P<0.05),但两组Syncytin-1 mRNA表达水平比较差异无统计学意义(P>0.05)。多因素logistic回归分析结果显示,较高的DLX3 mRNA表达水平是促进GDM发生的危险因素(OR=8.142,P=0.004)。结论DLX3是促进GDM发生的危险因素,其可能参与了GDM及胎盘钙化的发生。

Objective To explore the expressions of distal-less homeobox 3(DLX3)and Syncytin-1 mRNA in placental calcified tissues of patients with gestational diabetes mellitus(GDM)and their significances.Methods Thirty GDM patients admitted to Department of Obstetrics,the People′s Hospital of Guangxi Zhuang Autonomous Region from September 2015 to September 2018 were selected as the observation group,and 30 healthy pregnant women were selected as the control group during the same period.The general clinical data and the expression levels of DLX3 and Syncytin-1 mRNA(detected by real-time quantitative polymerase chain reaction)in placental calcified tissues were compared between the two groups,and the influencing factors of the occurrence of GDM were explored.Results The weight gain during pregnancy in the observation group was(11.15±4.03)kg,and that in the control group was(13.23±2.98)kg,and the difference between the two groups was statistically significant(t=2.069,P=0.044).There were 3 cases of macrosomia in the observation group(10.00%)and 1 case in the control group(3.33%),and there was no significant difference between the two groups(χ^(2)=0.268,P=0.605).The expression level of DLX3 mRNA in the observation group was significantly higher than that in the control group(P<0.05),but there was no significant difference in the expression level of Syncytin-1 mRNA between the two groups(P>0.05).Multivariate logistic regression analysis showed that higher DLX3 mRNA expression level was a risk factor for the occurrence of GDM(OR=8.142,P=0.004).Conclusion DLX3 is a risk factor for promoting the occurrence of GDM,and it may be involved in the occurrence of GDM and placental calcification.