盐穗木转录因子HcSCL13基因的时空表达和胁迫响应特性

Study of the Promoter properties of the HcSCL13 Gene for the Transcription Factor of Halostachys caspica(Bieb.)C.A.Mey.

【目的】研究盐生植物盐穗木HcSCL13基因的时空表达和胁迫响应特性。【方法】将该基因全长启动子(2230 bp)及截短序列与GUS报告基因融合,检测遗传转化后植物的GUS组织化学染色及酶活力。【结果】在稳定整合HcSCL13启动子的转基因拟南芥中,从种子萌发到种苗形成的过程中,地上和地下部位均表现出较强的启动子活性;随着植物的生长发育,启动子活性表现出一定的组织特异性;HcSCL13基因启动子对光、盐及机械损伤等胁迫积极响应。启动子上游-1124-1450 bp是影响其活性的区域,而上游-1730-2230 bp为盐响应区域。【结论】盐穗木HcSCL13基因的表达具有时空特异性及对光、盐和机械损伤等因素的响应特性。

【Objective】To explore the spatiotemporal expression and stress response properties of HcSCL13 genes in Halophytes caspica(Bieb.)C.A.Mey.【Method】The full-length promoter(2,230 bp)and truncated sequence of the gene were fused to the GUS reporter to detect the GUS histochemical staining and enzyme vitality of genetically transformed plants.【Results】Both aerial and underground parts of PHcSCL13.GUS homozyous Arabidopsis showed strong GUS staining at the stage of seed germination and seedling.With the development of transgenic PHcSCL13.GUS plant,the promoter activity displayed spatial and temporal patterns.Meanwhile,the promoter of HcSCL13 gene was responsive to light,salt and mechanical damage by GUS staining and activity analysis.After transient transformation of different length promoter fragments into the tobacco,the results of GUS activity suggested that the region for-1,124-1,450 bp upstream of the HcSCL13 promoter was responsible for its activity,while-1,730-2,230 bp upstream of the promoter was the salt response region.【Conclusion】The above finding shows that HcSCL13 genes in Halophytes caspica(Bieb.)C.A.Mey.express space-time specificity and response properties to light,salt and mechanical damage,which lays the foundation in molecular mechanism.