富硒乳酸菌亚硒酸盐还原特性及产硒机理研究

Selenite reduction characteristics and selenium production mechanism of selenium-enriched lactic acid bacteria

以前期分离筛选自秦巴富硒区的富硒乳酸菌为材料,探索无机硒转化为纳米单质硒的转化合成机理。采用透射电镜和扫描电镜技术研究发现纳米硒在其在细胞胞内外均有分布,主要集中在胞外。研究富硒乳酸菌的不同细胞组分对亚硒酸盐的还原能力,结果显示富硒乳酸菌主要通过硫氧还蛋白还原酶和延胡索酸还原酶等的作用将亚硒酸盐还原为纳米硒单质。采用Zeta电位分析仪、X射线光电子能谱技术、傅里叶红外光谱技术分析纳米硒的特性,结果说明富硒乳酸菌转化产物为纳米单质硒,且其外包裹多糖、蛋白质等多种活性物质。

In order to explore the conversion and synthesis mechanism from inorganic selenium into nano-selenium,the selenium-enriched lactic acid bacterium isolated and screened from the selenium-enriched area in Qinba moutain were used as materials.The results of transmission electron microscopy(TEM) and scanning electron microscopy(SEM) analysis showed that selenium nanoparticles were distributed both inside and outside the cell,mainly outside the cell.The reduction ability of different cell components of selenium-enriched lactic acid bacteria toward selenite was studied,and the results showed that selenite was reduced to nano-selenium by thioredoxin reductase and fumarate reductase.The characteristics of nano-selenium were analyzed by Zeta potential analyzer,X-ray photoelectron spectroscopy and Fourier infrared spectroscopy.The results showed that the transformation products of selenium-enriched lactic acid bacteria were nano-selenium,which were coated with polysaccharide,protein and other active substances.