COX-2和PTEN在中耳胆脂瘤中的表达及相关性研究

Expressions and correlation of COX-2 and PTEN in middle ear cholesteatoma

目的检测中耳胆脂瘤中环氧化酶-2(COX-2)和磷酸酶与张力蛋白同源物(PTEN)的表达,探讨COX-2和PTEN在中耳胆脂瘤发病机制中的作用。方法收集2018年5月—2019年4月就诊于西南医科大学附属医院手术的中耳胆脂瘤标本35例、中耳肉芽标本20例,随机收集上述患者的外耳道正常皮肤10例作为对照组。应用免疫组化染色、免疫印迹法(Western blot)检测3组标本中COX-2和PTEN的表达,运用Pearson相关性分析检验COX-2和PTEN在中耳胆脂瘤表达的相关性。结果COX-2在中耳胆脂瘤组中的免疫及蛋白表达明显强于中耳肉芽组及对照组,PTEN在中耳胆脂瘤组中的免疫及蛋白表达明显弱于中耳肉芽组及对照组,差异均具有统计学意义(P<0.05),且中耳胆脂瘤组中COX-2和PTEN的表达呈负相关(P<0.05)。结论COX-2表达增加和PTEN表达减少与中耳胆脂瘤的发病密切相关,二者可能通过PI3K/Akt信号通路参与了中耳胆脂瘤的发生发展。

Objective To expore the roles of cyclooxygenase-2(COX-2)and phosphatase and tensin homology deleted on chromosome ten(PTEN)in the pathogenesis of middle ear cholesteatoma via detecting the expressions and correlation of COX-2 and PTEN.Methods Specimens of middle ear cholesteatoma(n=35),middle ear granuloma(n=20)and normal external auditory canal skin(control group,n=10)were obtained from the patients undergoing surgical treatment.The expressions of COX-2 and PTEN in the specimens were detected by immunohistochemical staining and western blotting,and the correlation between the expression of COX-2 and PTEN in middle ear cholesteatoma was explored by Person correlation analysis.Results The expression of COX-2 in the cholesteatoma group was significantly stronger than those in the granuloma group and the control group,while the expression of PTEN in the cholesteatoma group was significantly weaker than those in the granuloma group and the control group,and the differences were all statistically significant(P<0.05).Meanwhile,the expressions of COX-2 and PTEN in the cholesteatoma group had a significant negative correlation(P<0.05).Conclusion The increased expression of COX-2 and the decreased expression of PTEN are closely related to the incidence of cholesteatoma in the middle ear,which indicates that both COX-2 and PTEN may be involved in the occurrence and development of middle ear cholesteatoma through PI3K/Akt signaling pathway.