肿瘤坏死因子-α基因多态性与复发脑梗死患者阿司匹林不耐受遗传易感性的关系

TNF-αgene polymorphism and genetic susceptibility to aspirin intolerance in patients with recurrent cerebral infarction

目的探讨肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)基因rs1799964,rs1800630位点多态性与复发脑梗死患者阿司匹林不耐受遗传易感性的关系。方法纳入2020年1月-2020年12月本院收治的80例复发脑梗死患者,其中阿司匹林不耐受组19例,阿司匹林耐受(对照)组61例;采用美国ABI公司生产的3730XL测序仪对样本TNF-α基因rs1799964,rs1800630位点进行基因测序分型。结果(1)入组人群TNF-α基因rs1799964,rs1800630位点基因型分布符合Hardy-Weinberg平衡定律(P>0.05);(2)阿司匹林不耐受组rs1799964位点C等位基因频率高于对照组(34.21%VS 16.39%,P=0.018),rs1800630位点A等位基因频率也高于对照组(28.95%VS 11.48%,P=0.01),其中rs1800630位点A等位基因(OR=2.579,P=0.046)可能是阿司匹林不耐受的独立危险因素。结论TNF-α基因rs1799964位点C等位基因及rs1800630位点A等位基因可能是复发脑梗死患者阿司匹林不耐受的易感因素。

Objective To explore the relationship between the polymorphisms of TNF-a(rs1799964 and rs1800630)and susceptibility of aspirin intolerance in patients with recurrent cerebral infarction.Methods Eighty patients with recurrent ischemic stroke treated in our hospital from January 2020 to December 2020 were included.Nineteen patients were included in aspirin intolerance group and 61 patients were included to non-aspirin intolerance group.The 3730 XL sequencer produced by ABI Incorporation was applied to detect the sequence of rs1799964 and rs1800630.Results(1)The distribution of TNF-αallele rs1799964 and rs1800630 in the enrolled population complies with Hardy-Weinberg equilibrium law(P>0.05).(2)The frequency of the C allele at rs1799964 in the aspirin intolerance group was higher than that in the control group(34.21%VS 16.39%,P=0.018),and the frequency of the A allele at rs1800630 in the non-aspirin intolerance group was also higher than that in the control group(28.95%VS 11.48%,P=0.01),of which the A allele at rs1800630(OR=2.579,P=0.046)may be an independent risk factor for aspirin intolerance.Conclusion TNF-αgene rs1799964 locus C allele and rs1800630 locus A allele may be susceptible factors of aspirin intolerance in patients with recurrent cerebral infarction,suggesting that TNF-αgene can be used as a biomarker of aspirin intolerance susceptibility in patients with recurrent ischemic stroke.