摘要
目的研究西红花Crocus sativus L.柱头的化学成分及其抗炎活性。方法西红花95%乙醇提取物采用D101型大孔树脂、硅胶、Sephadex LH-20和HPLC等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。用LPS诱导的小鼠RAW 246.7细胞炎症模型评价化合物抗炎活性。结果从中分离得到14个化合物,分别鉴定为(2E,4E)-2-甲基-6-羰基-2,4-庚二烯酸-1-O-β-D-吡喃葡萄糖酯(1)、(2E,4E)-2-甲基-6-羰基-2,4-庚二烯酸甲酯(2)、山柰酚(3)、单侧西红花酸龙胆二糖酯(4)、(2E,4E)-2-甲基-6-羰基-2,4-庚二烯酸龙胆二糖酯(5)、(2E,4E)-2-甲基-6-羰基-2,4-庚二烯酸(6)、crocusatin M(7)、crocusatin K(8)、3,4-二羟基丁酸(9)、对羟基苯甲酸(10)、山柰酚-7-O-β-D-葡萄糖苷(11)、山柰酚-3-O-β-D-葡萄糖苷(12)、柚皮素-7-O-β-D-葡萄糖苷(13)、柚皮素-4′-O-β-D-葡萄糖苷(14)。化合物3、11可降低LPS诱导RAW 264.7细胞NO的释放量。结论化合物1是新化合物,化合物2是新天然产物,化合物6、8、13~14为首次从该属植物中分离得到,化合物3、11具有一定抗炎活性。
AIM To investigate the chemical from the stigma of Crocus sativus L.and their anti-inflammatory activities.METHODS The 95% ethanol extract from the stigma of C.sativus was isolated and purified by D101,silica,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-inflammatory activities were evaluated by LPS induced RAW 264.7 inflammatory cell model.RESULTS Fourteen compounds were isolated and identified as(2E,4E)-2-menthyl-6-oxohepta-2,4-dienoicacid-β-D-glucosyl ester(1),(2E,4E)-2-menthyl-6-oxohepta-2,4-dienoicacid-methyl ester(2),kaempferol(3),crocetin-β-D-gentiobiosyl ester(4),(2E,4E)-2-methyl-6-oxohepta-2,4-dienoicacid-β-D-gentiobiosyl ester(5),(2E,4E)-2-methyl-6-oxohepta-2,4-dienoic acid(6),crocusatin M(7),crocusatin K(8),3,4-dihydroxybutyric acid(9),p-hydroxybenzoic acid(10),kaempferol-7-O-glucoside.(11),kaempferol-3-O-glucoside(12),naringenin-7-O-glucoside(13) and naringenin-4′-O-glucoside(14).Compounds 3 and 11 could inhibit the production of NO by LPS-induced RAW 264.7 cells.CONCLUSION Compound 1 is a new compound.Compound 2 is a new natural product.Compounds 6,8,13-14 are isolated from genus Croucus for the first time.Compounds 3 and 11 have anti-inflammatory activities.
作者
童鑫
洪吟秋
张涛
童应鹏
王平
邹忠梅
TONG Xin;HONG Yin-qiu;ZHANG Tao;TONG Ying-peng;WANG Ping;ZOU Zhong-mei(College of Pharmacy,Zhejiang University of Technology,Hangzhou 310024,China;Institute of Medical Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China;Natural Products and Health Products Institute,Institute for Advanced Study of Taizhou University,Taizhou 318000,China)
出处
《中成药》
CAS
CSCD
北大核心
2022年第4期1142-1147,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金青年基金项目(81703688)
国家“重大新药创制”科技重大专项资助项目(2019ZX09735002)
浙江省科技计划项目国际科技合作一带一路专项项目(2017C04009)
政府间国际科技创新合作重点专项(2017YFE0130100)。
关键词
西红花
柱头
化学成分
分离鉴定
抗炎活性
Crocus sativus L.
stigma
chemical constituents
isolation and identification
anti-inflammatory activities