胃癌组织中CapG、Prox-1、TrkB的表达与胃癌淋巴结转移及预后的关系

Relationship between the expression of CapG, Prox-1, TrkB and lymph node metastasis and prognosis of gastric cancer

目的探究胃癌组织中巨噬细胞加帽蛋白G(CapG)、同源盒基因转录因子1(Prox-1)、酪氨酸激酶受体B(TrkB)的表达与胃癌淋巴结转移及预后的关系。方法回顾性选取2018年1月至2020年12月安徽医科大学第三附属医院收治的100例胃癌患者作为研究对象,根据术前有无胃癌淋巴结转移将患者分为转移组(n=45)与未转移组(n=55)。术前对患者胃癌组织中CapG、Prox-1、TrkB表达情况予以检测,并对比转移组与未转移组阳性表达率。采用受试者工作特征(ROC)曲线对不同分子标志物表达情况对胃癌淋巴结转移及预后的影响进行分析。结果转移组CaPG、Prox-1、TrkB阳性表达率分别为84.44%、66.67%、75.56%,均显著高于未转移组(32.73%、12.73%、27.27%),差异均有统计学意义(P<0.05)。联合检测对胃癌淋巴结转移ROC曲线下面积高于单一指标检测,其中CapG、Prox-1、TrkB联合检测特异度最高,为97.42%,CapG联合TrkB敏感度最高,为89.52%;CapG、Prox-1、TrkB表达阳性患者生存率明显低于表达阴性患者,差异有统计学意义(P<0.05);CapG表达阳性是胃癌患者预后独立影响因素。结论胃癌组织中CapG、Prox-1、TrkB阳性表达提示患者存在胃癌淋巴结转移风险,预后差,生存率低,应予以高度重视。

Objective To investigate the relationship between the expression of CapG,Prox-1 and TrkB in gastric cancer and lymph node metastasis and prognosis.Methods A total of 100 gastric cancer patients admitted to theThird Affiliated Hospital of Anhui Medical University from January 2018 to December 2020 were retrospectively selected as the study subjects.The patients were divided into metastatic group(n=45)and non-metastatic group(n=55)according to the preoperative lymph node metastasis of gastric cancer.The expression of CapG,Prox-1 and TrkB in gastric cancer tissues of patients were detected before surgery,and the positive expression rate of the metastatic group and the non-metastatic group was compared.The receiver operating characteristic curve(ROC curve)was used to analyze the influence of the expression of different molecular markers on the lymph node metastasis and prognosis of gastric cancer.Results The positive expression rates of CaPG,Prox-1,and TrkB in the metastatic group were 84.44%,66.67%,and 75.56%,respectively,which were significantly higher than those in the non-metastatic group(32.73%,12.73%,27.27%),and the differenced were statistically significant(P<0.05).The area under the ROC curve of combined detection for gastric cancer lymph node metastasis was higher than that of a single index.The combined detection of CapG,Prox-1,and TrkB had the highest specificity at 97.42%,and the sensitivity of CapG combined with TrkB was the highest at 89.52%;CapG,Prox-1.The survival rate of patients with positive TrkB expression was significantly lower than that of patients with negative expression,and the difference was statistically significant(P<0.05);positive CapG expression was an independent prognostic factor in patients with gastric cancer.Conclusion The positive expression of CapG,Prox-1,and TrkB in gastric cancer tissues suggests that patients have the risk of gastric cancer lymph node metastasis,the prognosis is poor,and the survival rate is low,which should be taken seriously.