摘要
RNA干扰(RNA interference,RNAi)技术是现代农作物病虫害防治中的一项具有前景的生物技术,其专一性、高效性、环境友好等特点使其成为能够替代传统依赖化石能源农药的可行性方案,也是现代农业绿色生产中重要的生物技术之一。根据双链RNA(dsRNA)的合成和递送方式的不同,可以将RNAi技术在农业上的应用形式分成3类,分别为植物宿主诱导的基因沉默(Host-induced gene silencing,HIGS)、微生物载体介导的基因沉默(Vector-mediated gene silencing,VMGS)和外源dsRNA诱导的基因沉默(Exogenous dsRNA-induced gene silencing,EdIGS)。基于RNAi技术开发的生物农药在农业病虫害防治中具有广阔的应用前景。通过综述植物RNAi机制、RNAi技术的3种应用形式,梳理其在作物保护中应用的研究进展。
RNA interference(RNAi)technology is a promising biotechnology in the prevention and control of crop pests and diseases.Its specificity,high efficiency and environmental friendliness make it a feasible solution to replace traditional fossil energy pesticides,and also one of the important biological technologies in the green production of modern agriculture.According to the synthesis and delivery methods of dsRNA,RNAi technology can be applied in agriculture with three forms:host-induced gene silencing(HIGS),vector-mediated gene silencing(VMGS)and Exogenous dsRNA-induced gene silencing(EdIGS).RNAi-based bio-pesticides have a broad application prospect in agricultural pathogen and pest control.Here,the mechanism of plant RNAi,three forms of RNAi technology and their application in crop protection are reviewed.
作者
莫芹
徐莉莉
吕贝贝
MO Qin;XU Lili;LÜ Beibei(Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China;Shanghai Agricultural Technology Extension Service Center,Shanghai 201799,China)
出处
《上海农业学报》
2022年第2期136-142,共7页
Acta Agriculturae Shanghai
基金
上海市鲜食玉米产业体系建设[沪农科产字(2021)第10号]
上海市科委一带一路项目(20310750500)
上海市农业科学院助跑项目(ZP21211)
上海市农业科学院卓越团队[农科创2017(B-07)]
上海市农业科学院科研专项[农科应基2021(09)]
生物育种和生物安全评价及国际合作平台能力提升建设——农科平台[2021(08)]。
