水溶性二铁六羰基配合物与生物相关分子的相互作用及其促进一氧化碳释放

Interactions of a Water-Soluble Diiron Hexacarbonyl Complex with Biologically Relevant Molecules and Their Promotion in CO-Release

二铁六羰基配合物[Fe_(2)(μ‑SCH_(2)R)_(2)(CO)_(6)](R=CH(OH)CH_(2)(OH),1)是一个水溶性且能够释放一氧化碳的分子(CORM),我们应用各种光谱技术研究了其与血红蛋白(Hb)、肌红蛋白(Mb)、牛血清白蛋白(BSA)、谷胱甘肽(GSH)和DNA等生物分子的相互作用。红外光谱结果表明,蛋白质和谷胱甘肽均能促进配合物1分解释放CO。该CO释放过程符合一级动力学模型,其中谷胱甘肽促进CO释放的效率最高。紫外吸收光谱变化和荧光猝灭效应也表明这些生物相关分子与二铁羰基配合物之间存在相互作用。蛋白质和配合物1的CD光谱结果显示,配合物没有引起蛋白质的构象变化。pUC19质粒DNA与配合物1之间的作用表明该配合物不会引起DNA损伤。

In this study,a water-soluble diiron carbonyl complex,[Fe_(2)(μ-SCH_(2)R)_(2)(CO)_(6)](R=CH(OH)CH_(2)(OH),1),which has the potential as a CO-releasing molecule(CORM),was used to spectroscopically investigate its interaction with some biological molecules,such as hemoglobin(Hb),myoglobin(Mb),bovine serum albumin(BSA),glutathione(GSH),and DNA.The IR spectroscopic results showed that the proteins and GSH could promote the decomposition of complex 1 to release CO.All the CO-release progress followed the first-order kinetic model and GSH possessed the highest efficiency in promoting CO-release.UV absorption spectral variations and fluorescent quench effect also indicated the interactions between these biologically relevant molecules and the diiron carbonyl complex.CD spectra of the mixture of the proteins and complex 1 indicated that no conformational changes in the proteins are induced.The interactions between pUC19 plasmid DNA and complex 1 suggested that the complex could not cause DNA damage.