大豆球蛋白A1a亚基同源建模及抗原表位的预测

Homology modeling and prediction of the linear B-cell antigenic epitopes of Glycinin A1a subunits

为探究大豆球蛋白A1a(Glycinin A1a)亚基的线性B细胞表位,在美国国家生物信息技术中心(National Center of Biotechnology Information,NCBI)数据库中索引到Glycinin A1a亚基的氨基酸序列,利用一系列生物信息软件对该亚基进行一级结构、二级结构分析,预测出的线性B细胞表位为^(24)EQPQQN^(29)、^(40)KPDNRI^(45)、^(56)NPNNK^(60)、^(80)RRPSYTNG^(87)、^(114)PQQPQQRGQS^(123)、^(197)QEQGGHQSQKGKHQQEEENE^(216)、^(247)NEGED^(251)、^(267)PPTDEQQQRP^(276)、^(285)DEKPQCKGK^(293)、^(299)RPRGS^(303)。再使用SWISS-MODEL对Glycinin A1a亚基的三级结构完成同源建模,使用DiscoTope 2.0 Server对大豆球蛋白A1a亚基进行构象表位预测。这为进一步探究大豆致敏表位缩小了范围,为后期大豆球蛋白A1a亚基过敏原检测提供参考。

To explore the linear B-cell epitopes of Glycinin A1a subunits,the amino acid sequence of Glycinin A1a was indexed in National Center of Biotechnology Information(NCBI),then the primary and secondary structure of Glycinin A1a were analyzed by a series of bioinformatics software.The predicted linear B-cell epitopes are^(24)EQPQQN^(29),^(40)KPDNRI^(45),^(56)NPNNK^(60),^(80)RRPSYTNG^(87),^(114)PQQPQQRGQS^(123),^(197)QEQGGHQSQKGKHQQEEENE^(216),^(247)NEGED^(251),^(267)PPTDEQQQRP^(276),^(285)DEKPQCKGK^(293)and^(29)9 RPRGS^(303).In addition,the three-dimensional model of Glycinin A1a was built by SWISS-MODEL,and the DiscoTope 2.0 Server was used to make a fractal epitope prediction of Glycinin A1a subunit.This prediction narrows down the scope of soybean allergenic epitopes identification,and it provides a reference for the detection of allergens.