羧甲基纤维素诱导培养下罗伦隐球酵母的转录组差异分析

Differential analysis of the transcriptome of Cryptococcus laurentii under carboxymethyl cellulose induced culture

为探究罗伦隐球酵母(Cryptococcus laurentii)响应羧甲基纤维素(carboxymethyl cellulose,CMC)诱导培养的分子机理,为多糖诱导C.laurentii生理代谢相关基因的挖掘提供理论依据。该实验以不加CMC培养的C.laurentii为对照组,加0.5%(质量分数)CMC培养的C.laurentii为处理组(0.5%CMC),观察了CMC诱导培养对C.laurentii细胞生长形态变化。在此基础上,通过Illumina高通量测序技术,对2组酵母菌进行转录组测序,并采用生物信息学方法对数据分析。结果表明,添加0.5%(质量分数)CMC培养后,部分酵母细胞形态呈椭圆形,0.5%CMC诱导培养24~72 h,酵母细胞形态变化率显著(P<0.05)高于对照组。转录组数据分析表明,CMC诱导培养24 h,C.laurentii共有58个差异基因(differential genes,DEGs),其中55个为上调表达,3个为下调表达。DEGs被注释到24条GO(gene ontology)分类中和14条KEGG(Kyoto Encyclopedia of Genes and Genomes)通路中,分析结果表明,0.5%CMC诱导培养24 h C.laurentii涉及的DEGs功能主要与细胞能量代谢和生长、繁殖等有关。上述结果可以为多糖诱导培养对C.laurentii的生长影响及提高酵母拮抗效力分子机制的进一步研究提供科学依据,其可为C.laurentii形态变化相关基因挖掘提供理论基础,并为今后C.laurentii的商业化应用提供了一定的现实指导意义。

To investigate the molecular mechanism of Cryptococcus laurentii in response to carboxymethyl cellulose(CMC)induction culture,and to provide a theoretical basis for the excavation of genes related to polysaccharide-induced physiological metabolism of C.laurentii,C.laurentii cultured without CMC was set as the control group and C.laurentii yeast cultured with 0.5%CMC as the treatment group(0.5%CMC),and the morphological changes of CMC-induced culture on the cell growth of C.laurentii were observed.The transcriptomes of these two yeast groups were sequenced by Illumina high-throughput sequencing,and the data were analyzed by bioinformatics methods.The results showed that some yeast cells were oval in morphology after the addition of 0.5%CMC in culture,and the rate of morphological change of yeast cells was significantly(P<0.05)higher in 0.5%CMC induced culture for 24-72 h than that of the control group.Transcriptome data analysis showed that there were 58 differential expressed genes(DEGs)in C.laurentii at 24 h of CMC induction,of which 55 were up-regulated and three were down-regulated.DEGs were annotated into 24 GO classifications and 14 KEGG pathways,and the analysis showed that the functions of DEGs in C.laurentii were mainly related to cellular energy metabolism and growth and reproduction when induced by 0.5%CMC for 24 h.The results provide a scientific basis for further research on the effect of polysaccharide-induced culture on the growth of C.laurentii and the molecular mechanism to improve the antagonistic potency of yeast,which can provide a theoretical basis for the excavation of genes related to morphological changes in C.laurentii and practical guidance for the future commercial application of C.laurentii.