禽源空肠弯曲杆菌耐药性与致病性研究

Study on the Drug Resistance and Pathogenicity of Avian Campylobacter jejuni

为了解不同环节禽源空肠弯曲杆菌携带状况及其耐药性与致病性,从肉鸡养殖环节和屠宰环节采集600份样品并分离到241株空肠弯曲杆菌,采用微量肉汤稀释法和PCR方法对分离菌株进行耐药性和毒力基因检测。结果显示:分离菌株对环丙沙星、萘啶酸、四环素的耐药情况尤为严重,耐药率分别为97.75%、96.78%、95.18%;养殖环节分离菌株耐药性比屠宰环节严重,分离菌株对环丙沙星、四环素的耐药率高达100%,对萘啶酸的耐药率为98.67%,对阿奇霉素、红霉素、泰利霉素的耐药率较屠宰环节菌株高约35.00%;分离菌株除未检测到VirBII基因且neuB基因携带率较低外,其他14种毒力基因携带率都较高,有11种毒力基因的携带率达90%以上。将分离菌株接种小白鼠进行致病性试验,发现菌株对小白鼠具有一定致病性。结果表明,禽源弯曲杆菌耐药严重且有一定致病力,应加强对该菌的研究和控制。

In order to identify the carrying status,drug resistance and pathogenicity of avian Campylobacter jejuni(C.jejuni)in different processes,600 samples were collected from broiler breeding and slaughtering processes,and 241strains of C.jejuni were isolated to detect their drug resistance and virulence genes by micro broth dilution and PCR.The results showed that the isolates were strongly resistant to ciprofloxacin,naphthalidonic acid and tetracycline,with the drug resistance rates of 97.75%,96.78%and 95.18%,respectively;the resistance in breeding was more serious than that in slaughtering,with the resistance rate up to 100%to ciprofloxacin and tetracycline,98.67%to naphthyridine acid,and to azithromycin,erythromycin and talicamycin,35.00%higher than those in slaughtering;except VirBII gene that was not detected and neuB gene with lower carrying rate,the carrying rates of other 14 virulence genes were high,and above 90%in 11 genes.The isolates were vaccinated into mice for pathogenicity test.It was found that the isolates could produce certain pathogenicity to mice.In conclusion,C.jejuni was seriously resistant to drugs with certain pathogenicity,which should be further studied and controlled.