电针对东莨菪碱致谵妄大鼠海马单胺类神经递质的影响

Effects of Electroacupuncture on Monoamine Neurotransmitters in Hippocampus of Rat with Delirium Induced by Scopolamine

目的:通过观察海马单胺类神经递质变化探讨电针对东莨菪碱致谵妄模型大鼠认知功能的改善作用及机制。方法:将45只SD大鼠随机分为正常组、模型组、电针组,除正常组外,其余两组大鼠制备东莨菪碱诱导的谵妄模型,其后电针组行电针神庭、印堂穴干预,每次30 min,连续1周。对所有大鼠进行Morris水迷宫试验;采用ELISA法检测大鼠海马组织多巴胺(DA)、去甲肾上腺素(NE)的含量。结果:与模型组相比,电针组大鼠逃避潜伏期、第1次跨越平台时间明显缩短(P<0.05),120 s内跨越平台次数增多,差异有统计学意义(P<0.01);电针组较模型组海马DA、NE含量显著下降,差异具有统计学意义(P<0.05)。结论:电针神庭、印堂穴可降低谵妄大鼠海马内DA、NE含量,从而起到改善谵妄状态大鼠认知功能的作用。

Objective:To observe the effects of electroacupuncture at Shenting(DU24)and Yintang(Extra)acupoints on monoamine neurotransmitters in the hippocampus of rats with delirium,and explore the possible mechanism of electroacupuncture on the improvement of cognitive function in rats with delirium induced by Scopolamine.Methods:45 SD rats were randomly divided into three groups:noamal group,model group and electroacupuncture group.Except the normal group,the other two groups were prepared Scopolamine-induced model of delirium.After that,the electroacupuncture group was treated with electroacupuncture at Shenting and Yintang acupoints for 30 minutes each time for a consecutive 1 week.Then,Morris water maze test was carried out on all rats.The levels of dopamine(DA)and norepinephrine(NE)in hippocampus were measured by ELISA.Results:Compared with the model group,the escape latency and the first crossing time of the electroacupuncture group were significantly shortened(P<0.05),and the number of crossing times within 120 seconds was increased,and the difference was statistically significant(P<0.01);Compared with the model group,the contents of DA and NE in the hippocampus of the electroacupuncture group were significantly decreased,and the difference was statistically significant(P<0.05).Conclusion:Electroacupuncture at Shenting and Yintang acupoints can reduce the levels of DA and NE in the hippocampus of rats,and improve the cognitive function of rats in delirium state.