羧基功能化聚合物-量子点荧光微球探针的制备及其在槲皮素含量检测中的应用

Preparation of carboxyl functionalized fluorescent QDs embedded polymer microspheres for the determination of quercetin

目的:制备羧基功能化聚合物一量子点荧光微球,基于量子点荧光猝灭建立一种槲皮素低浓度检测的方法。方法:通过溶剂挥发法,采用聚甲基丙烯酸甲酯(PMMA)和聚马来酸酐-1-十八烯交替共聚物(PMA-ODE)对油溶性CdSe/ZnS核/壳量子点进行包裹制备羧基功能化聚合物一量子点荧光微球。基于槲皮素能够猝灭量子点荧光的特性,将羧基功能化聚合物一量子点荧光微球作为探针建立一种槲皮素低浓度检测的方法。研究槲皮素猝灭量子点荧光的机理,并优化检测条件。结果:经验证,该检测方法RSD小于3%,具有较好的精密度、重复性,回收率在99.9%~106.4%之间,检测限可达4.20×10^(-7)mol/L,具有广泛的应用前景。结论:该方法可用于槲皮素的含量测定。

Objective:Based on the fluorescence quench effect,a method of QCT detection at low concentration using QDs embedded polymer microspheres as probes were established.Methods:Aqueous carboxyl functionalized CdSe/ZnS QDs embedded polymer(PMMA/PMA-ODE) fluorescent microspheres were prepared by a solvent evaporation method.Based on the fluorescence quench effect,a method of QCT detection at low concentration using QDs embedded polymer microspheres as probes were established.The mechanism of QCT quenching on the fluorescence of microspheres was investigated Results:This QCT detection method showed higher precision,repeatability and recovery rate(99.9%~106.4%) with a detection limit of 4.2×10^(-7)mol/L,which has a broad application in QCT determination.Conclusion:This method can be used for the determination of quercetin.