唑来膦酸拮抗D-半乳糖诱导成骨细胞损伤的作用

Antagonistic Effect of Zoledronate on Osteoblast Injury Induced by D-galactose

目的:探究唑来膦酸对D-半乳糖导致成骨细胞损伤的拮抗作用。方法:以小鼠成骨细胞MC3T3-E1为研究对象,以MTT法观察不同浓度梯度(15、20、30 mg/mL)D-半乳糖及不同浓度梯度(10^(-8)、10^(-7)、10^(-6)、10^(-5)、10^(-4)、10^(-3)mol/L)唑来膦酸对成骨细胞活性的影响。以20 mg/mL D-半乳糖环境培养成骨细胞建立D-半乳糖导致成骨细胞损伤模型,观察不同浓度梯度(0.01、0.05、0.1、0.25、0.5μmol/L)唑来膦酸对D-半乳糖导致成骨细胞活性损伤的拮抗作用。结果:随着D-半乳糖浓度的升高(15、20、30 mg/mL),成骨细胞活性逐渐减弱。与对照组相比,较低浓度的(10^(-8)、10^(-7)mol/L)唑来膦酸干预下的实验组成骨细胞活性升高(P<0.05),而较高浓度的(10^(-5)、10^(-4)、10^(-3)mol/L)唑来膦酸干预下的实验组成骨细胞活性降低(P<0.0001)。以0.01、0.05μmol/L的唑来膦酸干预48 h后的实验组成骨细胞活性均较模型组升高(P<0.05)。结论:D-半乳糖能够导致成骨细胞活性下降。0.01、0.05μmol/L唑来膦酸对D-半乳糖导致成骨细胞活性损伤具有拮抗作用。

Objective:To investigate the antagonistic effects of Zoledronate on the inhibition of osteoblasts viability induced by D-galactose.Method:Taking mouse osteoblast MC3T3-E1 as the research object,the effects of different concentration gradients(15,20,30 mg/mL)of D-galactose and different concentration gradients(10^(-8),10^(-7),10^(-6),10^(-5),10^(-4),10^(-3)mol/L)of Zoledronate on osteoblast activity were observed by MTT method.Osteoblasts induced by D-galactose were cultured in 20 mg/mL D-galactose environment to establish an osteoblast injury model,and the antagonistic effect of Zoledronate on the activity of D-galactose induced osteoblasts was observed at different concentration gradient(0.01,0.05,0.1,0.25,0.5μmol/L).Result:With the increase of D-galactose concentration(15,20,30 mg/mL),the activity of osteoblasts decreased gradually.Compared with the control group,the osteoblast activity of the experimental groups with lower concentration(10^(-8),10^(-7)mol/L)of Zoledronate were increased(P<0.05),while the osteoblast activity of the experimental groups with higher concentration(10^(-5),10^(-4),10^(-3)mol/L)Zoledronate were decreased(P<0.0001).The osteoblast activity of the experimental groups were treated by 0.01 and 0.05μmol/L Zoledronate for 48 h were higher than that in the model group(P<0.05).Conclusion:D-galactose can decrease osteoblast activity.0.01 and 0.05μmol/L Zoledronate have antagonistic effect on D-galactose induced osteoblast activity injury.