猴头菇菌丝体富锗液体发酵培养及营养成分研究

Study on Liquid Fermentation and Nutrient Composition of Hericium erinaceus Mycelium Riched in Germanium

以猴头菇为研究对象,通过液体富锗培养获得富锗猴头菇菌丝体。采用高效液相色谱法(HPLC)、电感耦合等离子体质谱法(ICP-MS)等对富锗猴头菇菌丝体中粗多糖、蛋白质、氨基酸及有机锗进行定量分析。结果显示,当锗添加浓度为200 mg/L时,菌丝体粗多糖、蛋白质、氨基酸的含量及生物量较高,比对照组分别提高了112.12%、20.61%、30.59%、25.65%,各生物学指标的含量随着锗浓度的增加均呈现出先增加后减少的趋势;当锗添加浓度为400 mg/L时,有机锗含量为最高,比对照组提高了4974倍,有机锗含量随着锗浓度的增加而增加。该研究可为开发具有多种功效的猴头菇产品提供理论依据。

Hericium erinaceus was used as the research material and got Ge-rich mycelia by using of liquid cultivation in different concentration of Ge.The contents of crude polysaccharide,protein,amino acids and organic germanium in Hericium erinaceus mycelium were determined by high-performance liquid chromatography(HPLC)and inductively coupled plasma massspectrometry(ICP-MS).As compared with the control sample,the results showed that the contents of mycelium biomass,crude polysaccharide,protein and amino acids of Hericium erinaceus mycelium cultured in 200 mg/L germanium were increased by 112.12%,20.61%,30.59%and 25.65%,respectively.With the increase of germanium concentration,the contents of each biological index both increased at first and then decreased.When the concentration of germanium was 400 mg/L,the content of organic germanium was the highest,which was 4974 times higher than that of the control sample.The content of organic germanium increased with the increase of germanium concentration.The study can provide a theoretical basis for the development of Hericium erinaceus products with various functions.