膜联蛋白A2在病毒性社区获得性肺炎中的表达及意义

Expression and significance of annexin A2 in patients with viral community-acquired pneumonia

目的分析病毒性社区获得性肺炎(CAP)患者外周血单个核细胞(PMBCs)中膜联蛋白A2的表达差异,为病毒性肺炎治疗提供新思路。方法选取2016年1月至2018年1月我院住院治疗的病毒性CAP患者50例为病毒组,细菌性CAP患者47例为细菌组,同时选取健康体检者48例为对照组。采用实时荧光定量聚合酶链反应(qPCR)法测定患者PBMCs中膜联蛋白A2 mRNA的表达水平,采用蛋白免疫印迹(Western-blot)方法检测患者PBMCs中膜联蛋白A2蛋白的表达水平,分析病毒组中ANXA2表达的差异性。结果病毒组中单一病毒感染40例,混合病毒感染10例。单一病毒感染中流感病毒(30%)感染比例最高,其次为鼻病毒和冠状病毒,7例混合病毒感染包含了流感病毒。病毒组和细菌组的膜联蛋白A2 mRNA表达高于对照组,且病毒组膜联A2 mRNA的表达高于细菌组,有统计学意义(P<0.05),冠状病毒组ANXA2的mRNA和蛋白的表达较细菌性CAP组和对照组均增加,有统计学意义(P<0.05)。结论ANXA2可能通过更多的途径及机制参与病毒性肺炎的发生,ANXA2在冠状病毒感染中的作用可能更显著,ANXA2可成为病毒性CAP尤其是冠状病毒性CAP抗病毒治疗靶点。

Objective To investigate the expression of Annexin A2 in peripheral blood mononuclear cells of patients with viral community-acquired pneumonia,and provide new ideas for the antiviral treatment of viral pneumonia.Methods A total of 50 patients with viral CAP and 47 patients with bacterial CAP who were hospitalized in our hospital from January 2016 to January 2018 were selected as the virus group and bacteria group,respectively,and 48 healthy patients were selected as the control group.The quantitative real-time polymerase chain reaction(qPCR)method was used to determine the expression level of annexin A2 mRNA,and the western-blot method was used to detect the expression level of annexin A2 protein.Compare the relative expression levels of each group.Results There were 40 cases of single virus infection and 10 cases of mixed virus infection in the virus group.Influenza virus(30%)accounted for the highest proportion of single virus infections,followed by rhinoviruses and coronaviruses,and 7 mixed virus infections included influenza viruses.The expression of annexin A2 mRNA in the viral group and the bacterial group was higher than that in the control group,and the expression of the viral group was higher than that of the bacterial group.The difference was statistically significant(P<0.05).The expression of mRNA and protein of ANXA2 in the coronavirus group was higher than that of the bacterial CAP group and the control group.The difference was statistically significant(P<0.05).Conclusion ANXA2 may participate in the occurrence of viral CAP through more ways and mechanisms.The role of ANXA2 in coronavirus infection may be more significant.ANXA2 has the potential to become a target of antiviral therapy for viral CAP,especially coronavirus CAP.