贵州锌硒茶茶多糖的超声辅助提取工艺优化

Optimization of Extracting Polysaccharides from Guizhou Zinc-selenium Tea by Ultrasonic-assisted Methodology

【目的】探明贵州锌硒茶中茶多糖的最佳超声提取工艺,为贵州锌硒茶的生产应用提供参考。【方法】利用超声辅助水提取法从贵州凤冈锌硒茶中提取茶多糖,采用硫酸-苯酚法计算茶多糖提取率,在料液比、超声温度、超声时间和超声功率等单因素试验的基础上,利用响应面Box-behnken法对超声辅助提取茶多糖的工艺进行优化。【结果】最佳的超声辅助提取工艺条件为茶水料液比为1︰25、超声温度为70℃、超声时间为40 min、超声功率为150 W,在此工艺条件下茶多糖的提取率可达7.58%,比未超声提取样高42.50%。响应面优化试验交互作用表明,超声温度(A)、超声时间(B)和超声功率(C)对锌硒茶茶多糖的提取率影响程度为C>B>A;A、B和C两两间的交互作用对提取率的影响强弱程度为AB>BC>AC。【结论】超声辅助提取贵州锌硒茶中茶多糖的最佳工艺可以显著提高茶多糖提取率,操作简单,可行性强。

【Objective】The optimum ultrasonic extraction technology for polysaccharides from Guizhou Zn-Se tea is studied to provide a reference for the production and application of Guizhou Zn-Se tea.【Method】The ultrasonic-assisted water extraction method is used to extract polysaccharides from Guizhou Fenggang Zn-Se tea,and the extraction rate of tea polysaccharides is calculated by using phenol-sulfuric acid method.On the basis of single factor experiment such as material-liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power,the technology of ultrasonic-assisted extracting tea polysaccharides is optimized by adopting response surface Box-behnken method.【Result】The optimum ultrasonic-assisted extraction technology include 1︰25 of tea material-liquid ratio,70℃of ultrasonic temperature,40 min of ultrasonic time and 150 W of ultrasonic power.The extraction rate of tea polysaccharides is up to 7.58%under the optimum conditions,42.50%higher than that of samples without ultrasonic extraction.The interaction effect of response surface optimization experiment shows the influence of ultrasonic temperature(A),ultrasonic time(B)and ultrasonic power(C)on the extraction rate of polysaccharides in Zn-Se tea is C>B>A;The influence of the interaction between A,B and C on the yield is AB>BC>AC.【Conclusion】The optimum technology of ultrasonic-assisted extracting polysaccharides from Guizhou Zn-Se tea,with simple operation and strong feasibility,can significantly increase the extraction rate of tea polysaccharides.