大气压等离子体射流引发液相OH与H_(2)O_(2)的研究

Study on liquid phase OH and H_(2)O_(2) induced by atmospheric pressure plasma jet

大气压等离子体在生物医学领域有着巨大应用前景,等离子体引发的液相OH和H_(2)O_(2)具有重要的医学效应.明确等离子体工作参数与液相OH和H_(2)O_(2)生成之间的关系,进而实现液相OH和H_(2)O_(2)的调控,对于探明等离子体医学中关键作用机制具有重要的意义.笔者利用微秒脉冲直流高压等离子体射流作用去离子水,通过发射光谱研究不同工作参数(工作电压、脉冲宽度、脉冲频率以及工作气体组分)下等离子体射流关键气相激发态粒子的变化规律;采用荧光探针结合酶标仪测量了不同工作参数(工作电压、脉冲宽度、脉冲频率以及工作气体组分)下等离子体射流引发的液相OH和H_(2)O_(2)的绝对含量,并分析了它们之间的差异.结果表明:等离子体引发的液相OH可能主要是由高能粒子与水面的水分子反应生成的,气相OH溶解到水中的部分不是液相OH的主要来源;而液相H_(2)O_(2)则主要是由气相OH复合生成气相H_(2)O_(2)再溶解到水中形成的.

Atmospheric pressure plasma has excellent application prospects in the biomedical field.The liquid phase OH and H_(2)O_(2)induced by plasma have critical medical effects.Clarifying the relationship between the experimental parameters of plasma and the formation of OH and H_(2)O_(2)in the liquid phase and,furthermore,realizing the regulation of OH and H_(2)O_(2)in the liquid phase induced by plasma,is of great significance for investigating the key mechanism in plasma medicine.In this paper,the plasma jet driven by the microsecond pulsed DC high-voltage was used to treat deionized water,and the variation of the key gas phase excited particles in plasma jet under different experimental parameters(input voltage,pulse width,pulse frequency,and working gas composition) was studied by optical emission spectroscopy.The absolute content of OH and H_(2)O_(2)in the liquid phase induced by plasma jet was measured by fluorescence probe combined with Multifunctional Enzyme Marker under different experimental parameters(input voltage,pulse width,pulse frequency,and working gas composition),and the differences between them were compared.The results showed that the reaction between high-energy particles and water molecules on the water surface could mainly generate the OH in the liquid phase,and the gaseous OH dissolved in the water was not the primary source of the OH in the liquid phase.The liquid phase H_(2)O_(2)may be mainly formed by the gas phase OH compounding to the gas phase H_(2)O_(2)and then dissolving into the water.