三七总皂苷对局部脑缺血大鼠神经保护作用的研究

Study on Neuroprotection Effect of Total Panax Notoginseng Saponin in Rats with Local Cerebral Ischemia

目的:观察三七总皂苷对局部脑缺血大鼠的神经保护作用。方法:将80只雄性SD大鼠按随机数字表法分为空白对照组(A组,20只)和非空白大鼠组(60只),非空白大鼠组均需进行造模,造模成功后随机分组获得缺血模型组(B组)、阿替普酶治疗组(C组)和阿替普酶联合三七总皂苷治疗组(D组),每组20只。A组、B组均不进行治疗,C组给予注射用阿替普酶治疗,D组在C组基础上给予三七总皂苷治疗,均治疗5 d。比较4组首次治疗后24 h神经功能评分;比较4组治疗后脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)水平及血清肿瘤坏死因子-α (TNF-α)、白细胞介素-6 (IL-6)水平。结果:首次治疗后24 h,B组神经功能评分低于A组(P<0.05);C组、D组神经功能评分均高于B组(P<0.05),D组神经功能评分高于C组(P<0.05)。治疗后,B组脑组织MDA水平高于A组(P<0.05),脑组织SOD水平低于A组(P<0.05);C组、D组脑组织MDA水平均低于B组(P<0.05),脑组织SOD水平均高于B组(P<0.05);D组脑组织MDA水平低于C组(P<0.05),脑组织SOD水平高于C组(P<0.05)。治疗后,B组血清TNF-α、IL-6水平均高于A组(P<0.05);C组、D组血清TNF-α、IL-6水平均低于B组(P<0.05),D组血清TNF-α、IL-6水平均低于C组(P<0.05)。结论:三七总皂苷可以保护局部脑缺血大鼠的神经功能,抑制炎症反应与氧化应激反应。

Objective:To observe the neuroprotection effect of total Panax notoginseng saponin in rats with local cerebral ischemia. Methods:A total of 80 male SD rats were divided into the blank control group(Group A) and the non-blank rats group according to the random number table method,with 20 and 60 rats in each group respectively. The non-blank rats group were given model establishment. After successful establishment,the rats in the non-blank rats group were randomly divided into the ischemia model group(Group B),the alteplase treatment group(Group C) and the treatment with alteplase combined with total Panax notoginseng saponin group(Group D),with 20 rats in each group. There was no treatment given to Group A and B. Group C was given alteplase for injection,and Group D was additionally treated with total Panax notoginseng saponin based on the treatment of Group C,both groups were treated for five days. After the first treatment,the 24-hour neurological function scores in the four groups were compared. After treatment,the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) in the brain tissue,and the levels of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in serum in the four groups were compared. Results:At 24 hours after the first treatment,the neurological function score in Group B was lower than that in Group A(P<0.05);the neurological function scores in Group C and D were higher than that in Group B(P<0.05),and the score in Group D was higher than that in Group C(P<0.05). After treatment,the MDA level in brain tissue in Group B was higher than that in Group A(P<0.05),and SOD level was lower(P<0.05);the MDA levels in brain tissue in Group C and D were lower than that in Group B(P<0.05),and SOD levels in brain tissue were higher(P<0.05);the MDA level in brain tissue in Group D was lower than that in Group C(P<0.05),and SOD level was higher(P<0.05). After treatment,the levels of TNF-α and IL-6 in serum in Group B were higher than those in Group A(P<0.05);the levels of TNF-α and IL-6 in serum in Group C and D were lower than those in Group B(P<0.05),and the two levels in serum in Group D were lower than those in Group C(P<0.05). Conclusion: Total Panax notoginseng saponin can protect the neurological function in rats with local cerebral ischemia,and inhibit inflammatory responses and oxidative stress reactions.