基于MBBR技术的白酒窖底废水与低C/N生活污水协同处理系统的构建及功能微生物分析

Construction of a MBBR-based synergic treatment system for Baijiu pits bottom wastewater and low C/N domestic sewage and analysis on its functional microbes

为解决低C/N污水和高浓度白酒废水(HCBW)处理所面临的问题,采用移动床生物膜反应器(MBBR),构建了白酒窖底废水与模拟低C/N生活污水协同处理系统,以改性海绵填料和流化床填料分别探究HCBW作为反硝化外加碳源对低C/N生活污水处理的影响。结果表明:海绵填料协同处理系统(A1)对COD、NH_(4)^(+)-N、TN和色度的平均去除率分别为91.29%、99.08%、89.81%和80.66%,流化床填料系统(A2)的平均去除率分别为90.51%、98.58%、75.73%和76.07%,改性海绵填料的去除效果优于流化床填料;混合废水经过MBBR系统处理后,NH_(4)^(+)和TN得到了有效去除,出水中的醇类、硫酸盐和磷酸盐物质的相对比例有一定程度增加;协同处理系统A1和A2的硝化优势菌属均为Nakamurella、Nitrospira,反硝化优势菌属均为Amaricoccus、Dokdonella和Thermomonas,可能参与有机物去除的优势功能菌属均为Micropruina。通过功能预测得出:协同处理系统A1、A2中的主要代谢通路均为氨基酸代谢、碳水化合物代谢和能量代谢,主要的环境信息处理通路均为复制与修复和转译,主要的遗传信息处理通路均为膜运输。以上研究结果可为HCBW的资源化利用、低C/N生活污水处理提供参考。

In order to solve the problems faced by low C/N sewage and Baijiu pits bottom wastewater,a mobile bed biofilm reactor(MBBR)was used to build a collaborative treatment system for the liquor pits bottom wastewater and simulated low C/N sewage.Effect of HCLW as denitrification plus carbon source on low C/N wastewater treatment was investigated by using modified sponge filler or fluidized bed filler.The results showed the average removal rates of COD,ammonia nitrogen,total nitrogen and chroma by the modified sponge packing system(A1)were 91.29%,99.08%,89.81% and 80.66%,respectively;and by the fluidized bed packing system(A2)were 90.51%,98.58%,75.73% and 76.07%,respectively.The removal effect of the modified sponge filler was better than that of the fluidized bed filler.Ammonia nitrogen and total nitrogen could be effectively removed from the mixed wastewater after MBBR treatment,with a certain increase of alcohols,sulfate and phosphate contents in the effluent.Analysis results of 16S rRNA high-throughput sequencing technology showed that in the synergistic systems of A1 and A2,the dominant nitrifying bacteria were Nakamurella and Nitrospira;the dominant denitrifying bacteria were Amaricoccus,Dokdonella and Thermomonas;and the dominant functional bacteria for organic matter removal were Micropruina.Function prediction indicated that in the system A1 and A2,Amino Acid Metabolism,Carbohydrate Metabolism and Energy Metabolism were the top three metabolic pathways.Replication and Repair as well as Translation processed major environmental information.Membrane Transport was primarily responsible for the genetic information processing pathways.It was supposed to provide a technical support and theoretical basis for resource utilization of high-concentration Baijiu wastewater and treatment of low C/N domestic sewage.