摘要
牙槽骨缺损的再生治疗一直是口腔领域亟待攻克的难点。牙周组织工程技术的出现为解决牙槽骨缺损提供了新思路,其中良好的种子细胞是实现组织再生的关键要素。牙囊干细胞(DFSC)具有成骨分化潜能,且易于获取和保存,是极具开发前景的种子细胞。DFSC成骨分化过程涉及复杂的基因调控,其中非编码RNA(ncRNA)作为一类从DNA转录而来但不编码蛋白质的功能性RNA,已被证实可在表观遗传水平、转录水平及转录后水平参与调控干细胞成骨分化。探索ncRNA在DFSC成骨分化调控网络中的角色和作用机制,可为组织再生提供新的治疗策略。本文就DFSC成骨分化过程中微小RNA(miRNA)、长链非编码RNA(lncRNA)和环状RNA(circRNA)等各类ncRNA调控作用的研究进展作一综述。
Alveolar bone-defect regeneration has long been a challenge in the field of dentistry.The rising technology of periodontal tissue engineering provides a new strategy to solve it,and ideal seed cells are key elements of this technology.Dental follicle stem cells(DFSCs),as promising seed cells,have the potential of osteogenic differentiation,which are also easily accessible for cell culture and preservation.The osteogenic differentiation of DFSCs involves complex gene regulation.Noncoding RNA(ncRNA),as a functional RNA that is transcribed from DNA but does not encode protein,can reportedly regulate the osteogenic differentiation of stem cells at the epigenetic,transcription,and post-transcription levels.Exploring the role and mechanism of ncRNA in the regulatory network of DFSCs’osteogenic differentiation can provide novel therapeutic strategies for tissue regeneration.This review summarizes the advances in research on ncRNA during the osteogenic differentiation of DFSCs,including microRNA,long noncoding RNA,and circular RNA.
作者
洪娅娅
陈学鹏
姒蜜思
Hong Yaya;Chen Xuepeng;Si Misi(Dept.of Orthodontics,The Affiliated Hospital of Stomatology,School of Stomatology,Zhejiang University School of Medicine,Key Laboratory of Oral Biomedical Research of Zhejiang Province,Hangzhou 310006,China;Dept.of Implantology,The Affiliated Hospital of Stomatology,School of Stomatology,Zhejiang University School of Medicine,Key Laboratory of Oral Biomedical Research of Zhejiang Province,Hangzhou 310006,China)
出处
《国际口腔医学杂志》
CAS
CSCD
2022年第3期263-271,共9页
International Journal of Stomatology
基金
浙江省自然科学基金(LY18H140001)。
