难治性根尖周炎根管内及根尖外菌群的研究现状

Research status of the use of root canal and periapical microflora in refractory periapical periodontitis

难治性根尖周炎的致病因素包括根管内与根尖外的持续感染,两者的细菌种类既有联系又有差别。随着现代分子技术的发展,发现单一取样方式与传统细菌检测方法难以洞察根管中的所有细菌种类,而多种取样及检测方式的结合可更好地提高检测细菌的种类与数量。本文通过对近十年来难治性根尖周炎根管内及根尖外菌群研究文献的回顾,对不同取样方法及检测技术获得的难治性根尖周炎根管内外优势菌群的种类的比较,以及难治性根尖周炎的感染细菌来源等作一综述。

The pathogenic factors of refractory apical periodontitis include persistent intraradicular infection and extraradicular infection,both connectedly and differentially.With the development of modern molecular technology,it is found that the use of single sampling method and traditional bacterial detection methods makes it difficult to offer clear insight into all the bacterial species in the root canal.However,the combination of multiple sampling and detection methods can better improve the detection of species and populations of detected bacteria.Based on the review of literature on the intraradicular and extraradicular bacterial communities of refractory apical periodontitis in the last 10 years,this paper discusses the comparison of predominant microbial flora in and outside of the root canal of refractory apical periodontitis obtained by different sampling methods and detection techniques as well as the sources of infection microorganisms in refractory apical periodontitis.