氧化应激对断奶仔猪能量代谢和氨基酸表观消化率的影响

Effects of oxidative stress on energy metabolism and apparent digestibility of amino acids of the weaned piglets

选取12头、21日龄、平均体质量为(8.10±0.89)kg的健康二元杂(杜×长)断奶仔猪,于回肠末端实施“T”形瘘管手术,随机平均分为2组,处理组和对照组均饲喂基础饲粮,但处理组在基础日粮中根据每周体质量,每天拌入10g/kg剂量的D–半乳糖,建立D–半乳糖诱导断奶仔猪慢性氧化应激模型,探讨氧化应激对断奶仔猪血清生理生化指标、能量代谢和回肠末端氨基酸表观消化率的影响,试验期为28d。结果表明:D–半乳糖处理显著降低了断奶仔猪血清中总抗氧化能力、过氧化氢酶和总氧化物歧化酶的活性,显著提高了丙二醛的浓度;氧化应激显著降低了断奶仔猪血清尿素氮水平,而其他所测血清生化指标在两组间的差异均无统计学意义;氧化应激显著提高了断奶仔猪的氧气消耗量、二氧化碳产生量、产热量、呼吸熵和粪能;氧化应激显著或极显著降低了回肠末端必需氨基酸中的赖氨酸、蛋氨酸、苏氨酸、亮氨酸和异亮氨酸的表观消化率,同时也显著或极显著降低除脯氨酸和甘氨酸外的其他非必需氨基酸的表观消化率。使用10g/kg剂量的D–半乳糖建立断奶仔猪慢性氧化应激模型,断奶仔猪产生慢性氧化应激,不但降低了仔猪机体抗氧化酶系统活性和提高了氧化物水平,同时还抑制了仔猪能量代谢和回肠末端氨基酸消化水平,对断奶仔猪的生产产生不利影响。

This study aims to understand the effects of oxidative stress on serum biochemical indexes,energy metabolism and apparent digestibility of amino acids in terminal ileum.In the study,twelve healthy 21-day age weaned piglets(Duroc×Landrace)with an average weight of(8.10±0.89)kg were performed T cannula fit surgeon and then divided into 2 groups at random.The studied 2 groups were fed basal diets,and the body weight was weighed once a week.The treatment group was supplemented with D-galactose in the basal diet at a daily dose of 10 g/kg.We used D-galactose as a model to induce chronic oxidative stress in weaned piglets for 28 days.The results showed that,D-galactose supplementation significantly reduced the total antioxidant capacity,catalase and total superoxide dismutase activities in serum of weaned piglets,but significantly increased the concentration of malondialdehyde.Oxidative stress significantly reduced serum urea nitrogen levels in weaned piglets,while other serum biochemical indicators were not significantly different from the control group.Compared with the control group,oxidative stress significantly increased oxygen consumption,carbon dioxide production,heat production,respiratory quotient and fecal energy.Oxidative stress significantly or extremely significantly reduced the apparent digestibility of lysine,methionine,threonine,leucine and isoleucine among the terminal ileal essential amino acids,and it also significantly or extremely significantly reduced the apparent digestibility of non-essential amino acids except proline and glycine.A model of chronic oxidative stress in weaned piglets could be established using D-galactose at a dose of 10 g/kg.Chronic oxidative stress in weaned piglets not only reduced the activity of the antioxidant enzyme system and increases the level of oxidation,but also inhibited energy metabolism and terminal ileal amino acid digestion levels,which adversely affected the production of weaned piglets.