壳聚糖和纳米碳铜对铜绿微囊藻的抑制效果

Inhibition effects of chitosan and nano-carbon copper on Microcystis aeruginosa

选取0.5、1.0、2.0 mg/L的壳聚糖和25、50、100 mg/L的纳米碳铜分别加至铜绿微囊藻液中,检测藻液中藻细胞浓度和叶绿素a质量浓度,探讨壳聚糖和纳米碳铜对铜绿微囊藻的抑制效果;比较100 mg/L纳米碳铜与100、500 mg/L硫酸铜在除藻过程中藻液的pH值、溶解氧、铜离子的残留量,进一步验证纳米碳铜对铜绿微囊藻的抑制效果。结果表明:壳聚糖抑藻效果不明显;质量浓度为100 mg/L的纳米碳铜则具有明显的抑藻效果,144、360、480 h时,添加100 mg/L纳米碳铜组的藻细胞浓度显著低于其他组的,且72~480 h时,其藻细胞浓度随培养时间的增加而显著降低;48~240 h时,添加100 mg/L纳米碳铜组的藻液叶绿素a质量浓度和铜离子残留量均显著低于添加100、500 mg/L硫酸铜组的,且pH值变动小。这表明100 mg/L纳米碳铜可有效抑制铜绿微囊藻,且水体中铜离子的残留量较少,pH值变动小,可广泛用于水产养殖中。

The experiments of application of 0.5,1.0,2.0 mg/L chitosan and 25,50 and 100 mg/L nano-carbon copper to the Microcystis aeruginosa liquid,respectively,were set to detect the concentration of algae cells and chlorophyll a mass concentration in the algae liquid,and to investigate the inhibition effects of chitosan and nano-carbon copper on Microcystis aeruginosa.In the experiments,the pH value,dissolved oxygen and copper ion residue under the conditions of 100 mg/L nano-carbon copper and 100,500 mg/L copper sulfate were collected in the algae removal process to further verification of the inhibition effect of nano-carbon copper on Microcystis aeruginosa.The results showed that the algae inhibition effect of chitosan was not obvious,and the nano-carbon copper at 100 mg/L had obvious antibacterial effect,and the concentration of algal cells added to the 100 mg/L nano-carbon copper group was significantly lower than those of other groups at 144,360 and 480 h,and the concentration of algal cells decreased significantly over the increase of culture time at 72-480 h.At 48-240 h,the mass concentration of chlorophyll a and the residual amount of copper ion in algal liquid with the addition of 100 mg/L nano-carbon copper group were significantly lower than those of the 100 and 500mg/L copper sulfate groups,while small change in the p H value.Collectedly,this reveal that 100 mg/L nano-carbon copper can effectively inhibit Microcystis aeruginosa,the residual amount of copper ions and the pH change in water bodies are small,and it can be widely used in aquaculture.