活体骨内血管显像实验研究及临床意义初探

Experimental study and clinical significance of intravascular angiography in vivo

目的研究活体骨内血管显像方法,分析其临床意义,补充相关骨科疾患诊治依据。方法通过改进显影剂来增加局部浓度实现显影,以Lijianmin-Chengkun(LC)复合通路研究为基础,利用磁性微球理论,使用外壳为氨基(携负电荷)的Fe3O4磁性微球,吸附聚集离子型显影剂泛影葡胺(泛影酸根携正电荷),即通过改进显影剂的方法,使磁性微球携带显影剂,制成新的纳米粒子-磁影复合微粒,在外界磁场作用下,血液循环带来的磁影复合微粒不断在磁场区域血管内滞留聚集,磁场区域血管内磁性微球携带的显影剂浓聚达到显像浓度,实现活体骨内血管显像。并且通过调整两种试剂配比,可适度中和电荷凝结成小的集团,提高显影效率。由此分步进行了电镜实验、CT活体实验兔成像实验、实验兔组织学检查证实、CT活体人体成像试验。结果电镜实验:泛影葡胺,扫描电镜,微粒直径约20 nm。氨基Fe3O4磁性微球,扫描电镜,微粒直径约100 nm,分布较疏散均匀。两种试剂按一定比例混合后,中和电荷凝结成小的集团,但仍具备磁流体性能、强顺磁性。活体实验兔成像实验:捕捉到了理想的胫骨近端骨内血管成像。实验兔组织学检查证实:有磁场一侧胫骨近端内血管明显可见四氧化三铁分布,无磁场一侧未见。活体人体成像试验:捕捉到了理想的腓骨近端骨内血管成像。结论通过磁影复合微粒(磁性微球+泛影葡胺)新试剂的制作,在外界磁场作用下,达到活体骨内显影剂浓聚,在CT薄层扫描下可实现活体外径≥0.5 mm骨内血管成像。

Objective To study the method of intravascular angiography in vivo,analyze the clinical significance,and supply the basis of diagnosis and treatment of related orthopaedic diseases.Methods The development was realized by improving the developer to increase the local concentration.Based on the study of Lijianmin-Chengkun Complexes and using the theory of magnetic microspheres,Fe3O4 magnetic microspheres with amino(negatively charged)shell are used to adsorb the aggregated ionic developer meglumine diatrizoate(positively charged diatrizoate).That is,by improving the method of developer,the magnetic microspheres can carry the developer to make new nanoparticles magnetic imaging composite particles.Under the action of external magnetic field,the magnetic imaging composite particles brought by blood circulation continue to stay and gather in the blood vessels in the magnetic field area,and the developer carried by the magnetic microspheres in the blood vessels in the magnetic field area is concentrated to reach the imaging concentration,so as to realize in vivo intravascular vascular imaging.By adjusting the ratio of the two reagents,the charge can be neutralized and condensed into small groups to improve the development efficiency.Thus,the electron microscope experiment,CT in vivo experiment,rabbit imaging experiment,experimental rabbit tissue picture confirmation,CT in vivo human body(the author is a volunteer)imaging experiment were carried out step by step.Results Electron microscope experiment:meglumine diatrizoate,scanning electron microscope,the particle diameter is about 20 nm.Scanning electron microscope showed that the diameter of the magnetic microspheres was about 100 nm and the distribution was uniform.After the two reagents are mixed in a certain proportion,the neutralizing charge condenses into small groups,but it still has magnetohydrodynamic properties and strong paramagnetism.In vivo rabbit imaging experiment:the ideal intraosseous vascular imaging of the proximal tibia was captured.The tissue pictures of experimental rabbits confirmed that the distribution of Fe3O4 was obviously visible in the blood vessels in the proximal tibia on the side with magnetic field,but not on the side without magnetic field.In vivo human imaging experiment:the ideal intraosseous vascular imaging of the proximal fibula was captured.Conclusion Through the preparation of new reagent of magnetic imaging composite particles(magnetic microspheres+meglumine diatrizoate),the concentration of in vivo bone developer can be achieved under the action of external magnetic field,and the in vivo external diameter≥0.5mm can be achieved under CT thin-layer scanning.