麻风树JcWRI1基因克隆及功能分析

Cloning and functional analysis of JcWRI1 gene from physic nut

WRI1是AP2类转录因子的成员,在植物生长发育和脂肪酸合成途径中起着重要的调控作用。通过RT-PCR技术从麻风树中克隆了1个AP2家族基因,将其命名为JcWRI1。JcWRI1基因开放阅读框全长1137 bp,编码378个氨基酸。表达模式分析结果表明,在麻风树种子胚中没有检测到JcWRI1基因的表达,然而该基因在麻风树种子胚乳中高表达。亚细胞定位结果表明,JcWRI1基因编码1个核定位蛋白质。表型分析结果表明,提高JcWRI1基因的表达量不影响转基因水稻的生长发育,但是改变了转基因植株叶片、胚乳中脂肪酸组分的含量,并且提高了转基因水稻叶片、胚乳中的含油量。qRT-PCR结果表明,脂肪酸合成相关基因在JcWRI1转基因水稻中的相对表达量显著高于野生型。研究结果为将来研究JcWRI1基因在麻风树种子胚乳发育及油脂代谢途径中的功能提供了理论依据和新的基因资源。

WRI1 is a member of AP2 transcription factors and plays an important regulatory role in plant growth and development and fatty acid synthesis.In this study,an AP2 family gene was cloned from physic nut by RT-PCR and named JcWRI1.The open reading frame of JcWRI1 gene was 1137 bp in length,encoding 378 amino acids.The results of expression pattern analysis showed that the expression of JcWRI1 gene was not detected in the embryo,but it was highly expressed in the endosperm.The subcellular localization results indicated that the JcWRI1 gene encoded a nuclear localization protein.Phenotypic analysis revealed that the increase of JcWRI1 gene expression did not affect the growth and development of transgenic rice,but changed the fatty acid composition in the leaves and endosperm of transgenic plants and increased the oil content in the endosperm and leaves of transgenic rice.The results of qRT-PCR showed that the relative expression of fatty acid synthesis-related genes in JcWRI1 transgenic rice was significantly higher than that in wild-type rice.The results provide a theoretical basis and new genetic resources for further research on the function of JcWRI1 gene in the endosperm development and lipid metabolism pathway of physic nut.