基于转录组测序技术分析愈创木酚对禾谷镰刀菌的抑菌机制

Analysis on the antifungal mechanism of Fusarium graminearum treated by guaiacol based on transcriptome sequencing

植物源酚类化合物愈创木酚可以显著抑制禾谷镰刀菌的生长,本研究利用转录组测序分析愈创木酚对禾谷镰刀菌的作用机制,采用RNA-Seq技术从转录组水平分析禾谷镰刀菌在0.4μl/ml愈创木酚胁迫下的响应机制。结果显示,共筛选到905个差异表达基因表达量发生了变化,其中表达量上调的基因为464个,表达量下调的基因为441个。差异表达基因的COG、GO及Pathway功能分析发现,愈创木酚影响禾谷镰刀菌氧化应激反应、膜组分及离子运输途径。表明,愈创木酚处理后,禾谷镰刀菌的细胞膜完整性受到了破坏,Ca^(2+)运输途径受到破坏,同时愈创木酚作为抗氧化剂也能显著影响禾谷镰刀菌的氧化应激反应。

The plant derived phenolic compound guaiacol can significantly inhibit the growth of Fusarium graminearum.In this study,the action mechanism of guaiacol on F.graminearum was analyzed by transcriptome sequencing,and the response mechanism of F.graminearum under 0.4μl/ml guaiacol stress was analyzed by RNA-Seq technique at transcriptome level.The results showed that,905 differentially expressed genes(DEGs)were screened to be varied in expression quantity,of which 464 were up-regulated and 441 were down-regulated.Analysis on the function of COG,GO and pathway of DEGs showed that,guaiacol affected oxidative stress response,membrane components and ion transport pathways of F.graminearum.In conclusion,the integrity of the cell membrane and Ca^(2+) transport pathway of F.graminearum were damaged after treated with guaiacol,and guaiacol could also significantly affect the oxidative stress response of F.graminearum as an antioxidant.