C末端驱动蛋白1在肝癌中的表达及其临床意义

Expression of kinesin family member C1 in liver cancer and its clinical significance

目的探讨C末端驱动蛋白1(KIFC1)在肝癌中的表达及其临床意义。方法分别应用Oncomine、TPA及UALCAN数据库分析KIFC1在肝癌组织及正常肝脏组织中的表达情况,并探讨KIFC1表达与肝癌患者临床特征及预后的关系,通过STRING数据库分析与KIFC1相互作用的蛋白,并基于DAVID数据库进行基因本体(GO)功能注释及京都基因与基因组百科全书(KEGG)通路富集分析。结果通过Oncomine数据库检索出KIFC1相关研究346项,其中74项研究显示肿瘤组织和正常组织中KIFC1的表达差异有统计学意义,68项研究显示肿瘤组织中KIFC1高表达,6项研究显示肿瘤组织中KIFC1低表达,3项关于肝癌的研究显示KIFC1在肝癌组织中高表达,没有研究显示KIFC1在肝癌组织中低表达,Meta分析显示KIFC1在肝癌组织中的表达水平高于正常肝脏组织。TPA数据库中的免疫组化结果显示,KIFC1在正常肝脏组织中不表达,在肝癌组织中高表达。UALCAN数据库显示,肝癌组织中KIFC1的表达水平高于正常肝脏组织,且KIFC1的表达水平随着肿瘤分级及TNM分期的升高而升高,KIFC1低表达组肝癌患者的生存情况明显优于KIFC1高表达组患者(P﹤0.01)。STRING数据库显示与KIFC1相互作用的蛋白有8个,基于DAVID数据库分析这些基因参与4条信号通路,GO功能注释分析结果显示这些基因富集于11个细胞组分、3类分子功能和5类生物学过程。结论KIFC1在肝癌组织中表达水平较高,且其高表达与患者预后差有关,有望成为肝癌治疗的新靶点。

Objective To investigate the expression of kinesin family member C1(KIFC1)in liver cancer and its clinical significance.Method Oncomine,TPA,and UALCAN databases were used to analyze the expression of KIFC1 in liver cancer tissues and normal liver tissues,and the relationship between KIFC1 expression and the clinical characteristics and prognosis of liver cancer patients were also analyzed.The proteins interacting with KIFC1 were analyzed by the STRING database,and gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed based on the result from the DAVID database.Result Totally,346 KIFC1-related studies were retrieved from the Oncomine database,of which 74 studies showed a statistically significant difference in the expression of KIFC1 between tumor tissues and normal tissues.Among them,68 studies showed high expression of KIFC1 in tumor tissues,6 studies showed low expression of KIFC1 in tumor tissues,and 3 studies on liver cancer showed high expression of KIFC1 in liver cancer tissues,no study showed low expression of KIFC1 in liver cancer tissues.Meta-analysis presented that the expression of KIFC1 in liver cancer tissues was higher than that in normal liver tissues.The immunohistochemical results in the TPA database showed that KIFC1 was not expressed in normal liver tissues and was up-regulation in liver cancer tissues.The UALCAN database showed that the expression level of KIFC1 in liver cancer tissues was higher than that in normal liver tissues,and the expression level of KIFC1 increases with the increase of tumor grade and TNM stage.The survival of liver cancer patients in the KIFC1 down-regulation group was significantly better than that of the KIFC1 up-regulation group(P<0.01).The STRING database showed that 8 proteins interacted with KIFC1.Based on the DAVID database analysis,these genes are involved in 4 signaling pathways.The GO functional annotation analysis results show that these genes were enriched in 11 cellular components,3 molecular functions,and 5 biological processes.Conclusion KIFC1 is up-regulation in liver cancer tissues and associated with poor prognosis,which is expected to become a new and promising target for liver cancer treatment.